Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 71611..72244 Replicon chromosome
Accession NZ_CP010339
Organism Mycobacterium tuberculosis strain 22103

Toxin (Protein)


Gene name vapC Uniprot ID P9WFC0
Locus tag RN14_RS00360 Protein ID WP_003400580.1
Coordinates 71843..72244 (+) Length 134 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P0CW29
Locus tag RN14_RS00355 Protein ID WP_003400577.1
Coordinates 71611..71850 (+) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RN14_RS00345 (RN14_0068) 66909..68348 + 1440 WP_060811814.1 FAD-binding oxidoreductase -
RN14_RS20590 (RN14_0069) 68404..68565 + 162 WP_003899796.1 hypothetical protein -
RN14_RS00350 (RN14_0070) 68606..71544 + 2939 Protein_66 UPF0182 family protein -
RN14_RS00355 (RN14_0071) 71611..71850 + 240 WP_003400577.1 antitoxin VapB1 Antitoxin
RN14_RS00360 (RN14_0072) 71843..72244 + 402 WP_003400580.1 type II toxin-antitoxin system ribonuclease VapC1 Toxin
RN14_RS00365 (RN14_0073) 72296..74533 - 2238 WP_003899797.1 NADP-dependent isocitrate dehydrogenase -
RN14_RS00370 (RN14_0074) 74651..75220 - 570 WP_003400591.1 TetR/AcrR family transcriptional regulator -
RN14_RS00375 (RN14_0075) 75323..76234 + 912 WP_003899798.1 SDR family NAD(P)-dependent oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Pae0151-like, PIN domain

(36-124)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 134 a.a.        Molecular weight: 14339.44 Da        Isoelectric Point: 4.8887

>T52112 WP_003400580.1 NZ_CP010339:71843-72244 [Mycobacterium tuberculosis]
VDECVVDAAAVVDALAGKGASAIVLRGLLKESISNAPHLLDAEVGHALRRAVLSDEISEEQARAALDALPYLIDNRYPHS
PRLIEYTWQLRHNVTFYDALYVALATALDVPLLTGDSRLAAAPGLPCEIKLVR

Download         Length: 402 bp

>T52112 NZ_CP010339:71843-72244 [Mycobacterium tuberculosis]
GTGGATGAATGTGTAGTCGACGCGGCGGCCGTGGTTGACGCTCTCGCCGGCAAGGGCGCCAGCGCGATCGTTCTGCGCGG
TTTGCTCAAGGAGTCGATTTCTAACGCGCCGCATTTGCTGGACGCAGAGGTCGGACATGCACTCCGCCGCGCCGTGCTCA
GCGACGAAATCTCCGAAGAGCAGGCTCGCGCCGCGTTGGATGCCTTGCCTTATCTCATCGACAATCGTTACCCGCACAGC
CCACGACTGATCGAATACACATGGCAGCTAAGGCACAACGTCACGTTCTACGACGCCCTTTACGTCGCACTGGCCACCGC
ACTGGATGTCCCGCTGCTCACGGGCGACTCGCGGCTTGCGGCCGCGCCGGGCCTTCCGTGCGAAATCAAACTCGTTCGGT
GA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 8717.84 Da        Isoelectric Point: 8.5960

>AT52112 WP_003400577.1 NZ_CP010339:71611-71850 [Mycobacterium tuberculosis]
MATIQVRDLPEDVAETYRRRATAAGQSLQTYMRTKLIEGVRGRDKAEAIEILEQALASTASPGISRETIEASRRELRGG

Download         Length: 240 bp

>AT52112 NZ_CP010339:71611-71850 [Mycobacterium tuberculosis]
ATGGCTACCATTCAAGTTCGGGATTTGCCCGAAGATGTCGCCGAAACCTATCGACGGCGCGCCACCGCAGCGGGGCAGTC
GCTGCAGACGTATATGCGCACCAAGCTCATCGAAGGGGTGCGGGGCCGAGACAAGGCCGAGGCAATCGAGATCCTGGAAC
AGGCGCTCGCCAGCACTGCCAGCCCAGGCATCAGCCGGGAGACCATCGAGGCATCCCGGCGGGAGCTCAGGGGTGGATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4BR82

Antitoxin

Source ID Structure
AlphaFold DB A0A829CHM7

References