Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 2878739..2879376 Replicon chromosome
Accession NZ_CP010330
Organism Mycobacterium tuberculosis strain F28

Toxin (Protein)


Gene name vapC Uniprot ID P0DMV7
Locus tag RN05_RS13570 Protein ID WP_003413180.1
Coordinates 2878739..2879134 (-) Length 132 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WJ44
Locus tag RN05_RS13575 Protein ID WP_003413183.1
Coordinates 2879131..2879376 (-) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RN05_RS13530 2874142..2875353 + 1212 WP_009936235.1 alpha/beta hydrolase family protein -
RN05_RS13535 2875480..2876139 + 660 WP_003900846.1 LppA family lipoprotein -
RN05_RS13540 2876136..2876798 + 663 WP_003900848.1 hypothetical protein -
RN05_RS13545 2876795..2877073 + 279 WP_003901422.1 type II toxin-antitoxin system VapB family antitoxin -
RN05_RS13550 2877166..2877579 + 414 WP_003413164.1 PIN domain nuclease -
RN05_RS13555 2877618..2877875 + 258 WP_003413167.1 ribbon-helix-helix domain-containing protein -
RN05_RS13560 2877872..2878249 + 378 WP_003413174.1 type II toxin-antitoxin system VapC family toxin -
RN05_RS13565 2878265..2878639 - 375 WP_003413177.1 hypothetical protein -
RN05_RS13570 2878739..2879134 - 396 WP_003413180.1 type II toxin-antitoxin system toxin 23S rRNA-specific endonuclease VapC20 Toxin
RN05_RS13575 2879131..2879376 - 246 WP_003413183.1 type II toxin-antitoxin system antitoxin VapB20 Antitoxin
RN05_RS13580 2879787..2880206 - 420 WP_003413190.1 A24 family peptidase -
RN05_RS13585 2880218..2881027 - 810 WP_003413193.1 shikimate dehydrogenase -
RN05_RS13590 2881024..2882277 - 1254 WP_003413196.1 endolytic transglycosylase MltG -
RN05_RS13595 2882270..2882782 - 513 WP_003413197.1 Holliday junction resolvase RuvX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Endonuclease VapC20-like

(1-129)

Antitoxin

Ribbon-helix-helix protein, CopG

(11-49)


Sequences

Toxin        


Download         Length: 132 a.a.        Molecular weight: 14619.53 Da        Isoelectric Point: 6.9794

>T52097 WP_003413180.1 NZ_CP010330:c2879134-2878739 [Mycobacterium tuberculosis]
MIFVDTSFWAALGNAGDARHGTAKRLWASKPPVVMTSNHVLGETWTLLNRRCGHRAAVAAAAIRLSTVVRVEHVTADLEE
QAWEWLVRHDEREYSFVDATSFAVMRKKGIQNAYAFDGDFSAAGFVEVRPE

Download         Length: 396 bp

>T52097 NZ_CP010330:c2879134-2878739 [Mycobacterium tuberculosis]
ATGATCTTCGTCGACACGTCCTTCTGGGCTGCACTTGGCAACGCCGGCGACGCTCGGCACGGCACCGCGAAGAGGTTGTG
GGCCAGCAAACCGCCCGTGGTGATGACCTCCAACCATGTCCTGGGCGAGACCTGGACGCTGCTCAACCGGCGCTGCGGTC
ACCGCGCGGCGGTTGCCGCCGCTGCAATCCGCTTGAGCACCGTCGTTCGCGTCGAGCACGTAACAGCCGACCTGGAAGAG
CAGGCATGGGAATGGCTTGTACGCCACGACGAGCGAGAGTACTCGTTCGTCGATGCCACGAGCTTCGCGGTGATGCGAAA
GAAAGGCATCCAAAATGCGTATGCCTTCGATGGTGACTTCAGCGCGGCCGGATTTGTCGAGGTAAGACCCGAGTAG

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 9098.33 Da        Isoelectric Point: 5.6007

>AT52097 WP_003413183.1 NZ_CP010330:c2879376-2879131 [Mycobacterium tuberculosis]
MLVAYICHVKRLQIYIDEDVDRALAVEARRRRTSKAALIREYVAEHLRQPGPDPVDAFVGSFVGEADLSASVDDVVYGKH
E

Download         Length: 246 bp

>AT52097 NZ_CP010330:c2879376-2879131 [Mycobacterium tuberculosis]
ATGCTAGTGGCGTATATTTGCCATGTGAAAAGGCTGCAGATCTACATCGACGAAGACGTTGACCGAGCGCTTGCTGTTGA
AGCGCGACGGCGGCGGACGTCGAAGGCGGCGCTGATCCGGGAGTACGTCGCTGAGCACCTCCGGCAGCCGGGCCCAGATC
CGGTCGATGCCTTCGTCGGATCGTTCGTGGGGGAAGCCGACTTGTCCGCGTCCGTCGACGACGTGGTTTACGGAAAGCAC
GAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5WZ4
PDB 5WZF

Antitoxin

Source ID Structure
AlphaFold DB A0A7U4BW31

References