Detailed information of TA system TA052082

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2201601..2202304
• Replicon: chromosome
• Accession: NZ_CP010330
• Organism: Mycobacterium tuberculosis strain F28

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G0TLK7
• Locus tag: RN05_RS10245
• Protein ID: WP_003409778.1
• Coordinates: 2201601..2201930 (-)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: G0TLK8
• Locus tag: RN05_RS21385
• Protein ID: WP_003409780.1
• Coordinates: 2201927..2202304 (-)
• Length: 126 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RN05_RS10225	2197984..2199054	+	1071	WP_003899091.1	epoxide hydrolase EphB	-
RN05_RS10230	2199051..2199566	+	516	WP_003409718.1	flavin reductase family protein	-
RN05_RS10235	2199563..2200624	+	1062	WP_003899092.1	3,4-dihydroxy-2-butanone-4-phosphate synthase	-
RN05_RS10240	2200621..2201391	+	771	WP_003409775.1	SDR family oxidoreductase	-
RN05_RS10245	2201601..2201930	-	330	WP_003409778.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
RN05_RS21385	2201927..2202304	-	378	WP_003409780.1	type II toxin-antitoxin system antitoxin MazE5	Antitoxin
RN05_RS10255	2202301..2202891	-	591	WP_003409784.1	SEC-C domain-containing protein	-
RN05_RS10260	2202946..2204310	+	1365	WP_003903691.1	HNH endonuclease	-
RN05_RS10265	2204465..2204917	-	453	WP_003899095.1	lipoprotein	-
RN05_RS10270	2204981..2205382	+	402	WP_003409869.1	hypothetical protein	-
RN05_RS10275	2205375..2205557	-	183	WP_003409870.1	hypothetical protein	-
RN05_RS10280	2205671..2206021	-	351	WP_003899096.1	hypothetical protein	-
RN05_RS10285	2206032..2206934	-	903	WP_003899097.1	hypothetical protein	-
RN05_RS10290	2206955..2207146	-	192	WP_003409876.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-103)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 11787.82 Da
• Isoelectric Point: 8.5361

>T52082 WP_003409778.1 NZ_CP010330:c2201930-2201601 [Mycobacterium tuberculosis]
VTALPARGEVWWCEMAEIGRRPVVVLSRDAAIPRLRRALVAPCTTTIRGLASEVVLEPGSDPIPRRSAVNLDSVESVSVA
VLVNRLGRLADIRMRAICTALEVAVDCSR

Nucleotide

• Length: 330 bp

>T52082 NZ_CP010330:c2201930-2201601 [Mycobacterium tuberculosis]
GTGACCGCACTTCCGGCGCGCGGAGAGGTGTGGTGGTGTGAGATGGCTGAGATCGGTCGGCGACCAGTCGTCGTGCTGTC
GCGCGATGCCGCGATCCCTCGGCTGCGACGCGCACTTGTCGCGCCCTGCACCACGACCATCCGAGGGCTAGCCAGTGAGG
TTGTTCTTGAACCCGGTTCCGACCCGATCCCGCGCCGTTCCGCGGTGAATTTGGACTCAGTCGAAAGTGTCTCGGTCGCG
GTATTGGTGAATCGGCTTGGCCGCCTCGCCGACATCCGGATGCGCGCCATCTGCACGGCCCTCGAGGTCGCCGTCGATTG
CTCTCGATGA

Antitoxin

Protein

• Length: 126 a.a.
• Molecular weight: 13560.07 Da
• Isoelectric Point: 5.1519

>AT52082 WP_003409780.1 NZ_CP010330:c2202304-2201927 [Mycobacterium tuberculosis]
VKTARLQVTLRCAVDLINSSSDQCFARIEHVASDQADPRPGVWHSSGMNRIRLSTTVDAALLTSARDMRAGITDAALIDE
ALAALLARHRSAEVDASYAAYDKHPVDEPDEWGDLASWRRAAGDS

Nucleotide

• Length: 378 bp

>AT52082 NZ_CP010330:c2202304-2201927 [Mycobacterium tuberculosis]
GTGAAGACGGCCCGGTTGCAGGTGACGTTGCGCTGCGCGGTAGACCTCATCAATTCGTCCAGCGACCAATGTTTCGCCCG
TATCGAGCACGTTGCGTCCGATCAGGCGGATCCACGCCCCGGAGTGTGGCATAGTAGTGGCATGAATCGCATACGGCTGA
GCACTACTGTCGACGCCGCACTCTTGACAAGTGCGCGCGACATGCGGGCAGGAATCACTGACGCAGCCCTCATCGATGAG
GCCCTTGCGGCATTACTGGCTCGTCATCGATCGGCCGAGGTGGATGCCAGCTATGCGGCCTACGACAAGCACCCGGTCGA
TGAGCCCGACGAGTGGGGCGATCTAGCGTCGTGGCGGCGGGCGGCTGGAGACTCGTGA

Structures

Toxin

• AlphaFold DB: G0TLK7

Antitoxin

• AlphaFold DB: G0TLK8