Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) parDE/RelE-RHH
Location 130876..131423 Replicon plasmid pAPEC-O78-ColV
Accession NZ_CP010316
Organism Escherichia coli strain 789

Toxin (Protein)


Gene name parE Uniprot ID E9LLH3
Locus tag AC789_RS25480 Protein ID WP_000969996.1
Coordinates 131142..131423 (+) Length 94 a.a.

Antitoxin (Protein)


Gene name parD Uniprot ID E1QMW3
Locus tag AC789_RS25475 Protein ID WP_000079941.1
Coordinates 130876..131145 (+) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AC789_RS25455 126329..127528 + 1200 WP_000804064.1 tetracycline efflux MFS transporter Tet(A) -
AC789_RS25460 127560..128444 - 885 WP_000058717.1 EamA family transporter -
AC789_RS25465 128582..128989 - 408 WP_200889831.1 cysteine hydrolase -
AC789_RS25470 128978..130729 + 1752 Protein_124 Tn3-like element TnAs1 family transposase -
AC789_RS25475 130876..131145 + 270 WP_000079941.1 hypothetical protein Antitoxin
AC789_RS25480 131142..131423 + 282 WP_000969996.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
AC789_RS25485 131469..132317 + 849 WP_001057989.1 3'-5' exonuclease -
AC789_RS25490 132503..134383 + 1881 WP_001283335.1 colicin -
AC789_RS25495 134405..134740 - 336 WP_001080729.1 colicin 1A immunity protein -
AC789_RS25500 134869..135126 + 258 WP_000142431.1 hypothetical protein -
AC789_RS25505 135146..135736 - 591 WP_001350923.1 hypothetical protein -
AC789_RS25510 135733..135993 - 261 WP_000343091.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid sitABCD / tet(A) iroN / iroE / iroD / iroC / iroB / iucA / iucB / iucC / iucD / iutA 1..144859 144859


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-92)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 94 a.a.        Molecular weight: 10893.57 Da        Isoelectric Point: 8.4615

>T52040 WP_000969996.1 NZ_CP010316:131142-131423 [Escherichia coli]
MMEIFWTMLASQDRKRIREYIAEQNLMAAIELDERIGYSASSLAGQPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKV
YILRVLHTAQKWP

Download         Length: 282 bp

>T52040 NZ_CP010316:131142-131423 [Escherichia coli]
ATGATGGAGATATTCTGGACCATGCTGGCCAGCCAGGACAGGAAGCGCATTCGTGAGTACATTGCGGAGCAGAACCTGAT
GGCCGCCATTGAACTGGACGAACGGATTGGTTATTCAGCCAGCAGCCTGGCCGGGCAGCCGTATAAAGGCCGCAACGGTC
GTGTGGAAGGTACCCGGGAACTGGTTATTCACCCGCATTTTGTTCTGGTTTATGAGGTCGACAGCCAGTGGGGAAAAGTG
TATATCCTGCGTGTATTGCATACTGCACAGAAGTGGCCGTAG

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10252.58 Da        Isoelectric Point: 7.2057

>AT52040 WP_000079941.1 NZ_CP010316:130876-131145 [Escherichia coli]
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQHNTWFRDQVEAGRQQLERGDVLPHDMVESSAAAWRD
EMSRKVAGK

Download         Length: 270 bp

>AT52040 NZ_CP010316:130876-131145 [Escherichia coli]
ATGGCACAGGTTAATATGAGTTTAAGAATTGACGCTGAACTGAAGGATGCTTTTATGGCCGCTGCAAAAAGCATGGACCG
TAATGGCTCTCAGTTAATCCGGGATTTTATGCGCCAGACCGTGGAACGGCAGCATAATACCTGGTTCCGTGACCAGGTTG
AGGCAGGACGTCAGCAACTCGAGCGCGGCGATGTGCTTCCCCATGACATGGTCGAATCTTCCGCTGCTGCATGGCGGGAT
GAAATGAGCAGGAAAGTCGCCGGTAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB E9LLH3

Antitoxin

Source ID Structure
AlphaFold DB A0A629TXC5

References