Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3321428..3322082 Replicon chromosome
Accession NZ_CP010315
Organism Escherichia coli strain 789

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag AC789_RS16355 Protein ID WP_000244777.1
Coordinates 3321428..3321835 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag AC789_RS16360 Protein ID WP_000354046.1
Coordinates 3321816..3322082 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AC789_RS16335 3317385..3319118 - 1734 WP_000813212.1 single-stranded-DNA-specific exonuclease RecJ -
AC789_RS16340 3319124..3319834 - 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
AC789_RS16345 3319859..3320755 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
AC789_RS16350 3320867..3321388 + 522 WP_001055874.1 flavodoxin FldB -
AC789_RS16355 3321428..3321835 - 408 WP_000244777.1 toxin CptA Toxin
AC789_RS16360 3321816..3322082 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
AC789_RS16365 3322325..3323305 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
AC789_RS16370 3323382..3324041 - 660 WP_000250274.1 hemolysin III family protein -
AC789_RS16375 3324205..3324516 - 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
AC789_RS16380 3324561..3325994 + 1434 WP_001363803.1 6-phospho-beta-glucosidase BglA -
AC789_RS16385 3326051..3326794 - 744 WP_000951948.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T52033 WP_000244777.1 NZ_CP010315:c3321835-3321428 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T52033 NZ_CP010315:c3321835-3321428 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT52033 WP_000354046.1 NZ_CP010315:c3322082-3321816 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT52033 NZ_CP010315:c3322082-3321816 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References