Detailed information of TA system TA052030

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 2875422..2876047
• Replicon: chromosome
• Accession: NZ_CP010315
• Organism: Escherichia coli strain 789

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: AC789_RS14150
• Protein ID: WP_000911330.1
• Coordinates: 2875422..2875820 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: AC789_RS14155
• Protein ID: WP_000450524.1
• Coordinates: 2875820..2876047 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AC789_RS14130	2871300..2871500	+	201	WP_000383836.1	YpfN family protein	-
AC789_RS14135	2871610..2872308	-	699	WP_000679812.1	esterase	-
AC789_RS14140	2872382..2874397	-	2016	WP_000829335.1	tRNA cytosine(34) acetyltransferase TmcA	-
AC789_RS14145	2874412..2875275	-	864	WP_001267507.1	neutral zinc metallopeptidase	-
AC789_RS14150	2875422..2875820	-	399	WP_000911330.1	tRNA(fMet)-specific endonuclease VapC	Toxin
AC789_RS14155	2875820..2876047	-	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
AC789_RS14160	2876201..2876914	-	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
AC789_RS14170	2877127..2878161	-	1035	WP_001297320.1	outer membrane protein assembly factor BamC	-
AC789_RS14175	2878178..2879056	-	879	WP_001295469.1	4-hydroxy-tetrahydrodipicolinate synthase	-
AC789_RS14180	2879202..2879774	+	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
AC789_RS14185	2879774..2880244	+	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T52030 WP_000911330.1 NZ_CP010315:c2875820-2875422 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T52030 NZ_CP010315:c2875820-2875422 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT52030 WP_000450524.1 NZ_CP010315:c2876047-2875820 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT52030 NZ_CP010315:c2876047-2875820 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33