Detailed information of TA system TA052020

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 1796043..1796569
• Replicon: chromosome
• Accession: NZ_CP010315
• Organism: Escherichia coli strain 789

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1EXL1
• Locus tag: AC789_RS08795
• Protein ID: WP_000323025.1
• Coordinates: 1796043..1796330 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: S1F6D3
• Locus tag: AC789_RS08800
• Protein ID: WP_000534858.1
• Coordinates: 1796330..1796569 (-)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AC789_RS08755	1791067..1791282	-	216	WP_000839590.1	phage lysis protein EssD	-
AC789_RS08760	1792036..1792251	-	216	WP_000066484.1	cold shock-like protein CspB	-
AC789_RS08765	1792552..1792764	+	213	WP_000087756.1	cold shock-like protein CspF	-
AC789_RS29385	1792819..1792908	+	90	WP_120795389.1	hypothetical protein	-
AC789_RS08770	1793186..1793938	-	753	WP_001047135.1	antitermination protein	-
AC789_RS08775	1793952..1795001	-	1050	WP_001265198.1	DUF968 domain-containing protein	-
AC789_RS08780	1795003..1795281	-	279	WP_012304870.1	hypothetical protein	-
AC789_RS08785	1795348..1795599	-	252	WP_000980994.1	hypothetical protein	-
AC789_RS08790	1795816..1795971	-	156	WP_000813254.1	type I toxin-antitoxin system toxin HokD	-
AC789_RS08795	1796043..1796330	-	288	WP_000323025.1	type II toxin-antitoxin system mRNA interferase RelE	Toxin
AC789_RS08800	1796330..1796569	-	240	WP_000534858.1	type II toxin-antitoxin system antitoxin RelB	Antitoxin
AC789_RS27075	1796594..1796899	+	306	WP_001326990.1	hypothetical protein	-
AC789_RS08805	1797102..1797434	+	333	WP_001301033.1	protein FlxA	-
AC789_RS08810	1797871..1799185	-	1315	Protein_1678	ISNCY family transposase	-
AC789_RS30010	1799954..1800241	-	288	Protein_1679	hypothetical protein	-
AC789_RS08825	1800852..1801208	-	357	WP_001310834.1	class I SAM-dependent methyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1769405..1809687	40282

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-86)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-79)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11225.22 Da
• Isoelectric Point: 10.1967

>T52020 WP_000323025.1 NZ_CP010315:c1796330-1796043 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Nucleotide

• Length: 288 bp

>T52020 NZ_CP010315:c1796330-1796043 [Escherichia coli]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 9071.48 Da
• Isoelectric Point: 4.5230

>AT52020 WP_000534858.1 NZ_CP010315:c1796569-1796330 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Nucleotide

• Length: 240 bp

>AT52020 NZ_CP010315:c1796569-1796330 [Escherichia coli]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Structures

Toxin

• PDB: 4V7K
• PDB: 4V7J
• PDB: 4FXI
• PDB: 4FXE
• PDB: 2KC8
• PDB: 4FXH
• PDB: 2KC9

Antitoxin

• PDB: 4FXE
• PDB: 2KC8
• PDB: 2K29
• AlphaFold DB: A0A4V1CTS8