51991

Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) spoIISABC/SpoIISA-SpoIISB
Location 1327934..1328850 Replicon chromosome
Accession NZ_CP010314
Organism Bacillus subtilis subsp. subtilis strain 3NA isolate 1970 (Michel and Millet)

Toxin (Protein)


Gene name spoIISA Uniprot ID O34853
Locus tag RP72_RS07045 Protein ID WP_003244695.1
Coordinates 1328104..1328850 (-) Length 249 a.a.

Antitoxin (Protein)


Gene name spoIISB Uniprot ID G4EXD8
Locus tag RP72_RS07040 Protein ID WP_003232646.1
Coordinates 1327934..1328104 (-) Length 57 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RP72_RS07010 1324797..1325126 + 330 WP_003232660.1 phage-like element PBSX protein XkdW -
RP72_RS07015 1325123..1325287 + 165 WP_003232658.1 XkdX family protein -
RP72_RS07020 1325331..1326170 + 840 WP_003245597.1 phage-like element PBSX protein XepA -
RP72_RS07025 1326223..1326492 + 270 WP_003232655.1 hemolysin XhlA family protein -
RP72_RS07030 1326505..1326768 + 264 WP_003232653.1 phage holin -
RP72_RS07035 1326781..1327674 + 894 WP_003245230.1 N-acetylmuramoyl-L-alanine amidase -
RP72_RS23230 1327711..1327848 - 138 WP_003232648.1 three component toxin-antitoxin-antitoxin system antitoxin SpoIISC -
RP72_RS07040 1327934..1328104 - 171 WP_003232646.1 three component toxin-antitoxin-antitoxin system antitoxin SpoIISB Antitoxin
RP72_RS07045 1328104..1328850 - 747 WP_003244695.1 toxin-antitoxin-antitoxin system toxin SpoIISA Toxin
RP72_RS07050 1328960..1329961 - 1002 WP_003232642.1 inorganic phosphate transporter -
RP72_RS07055 1329974..1330591 - 618 WP_003218470.1 DUF47 domain-containing protein -
RP72_RS07060 1330867..1332183 - 1317 WP_003244819.1 serine/threonine exchanger -
RP72_RS07065 1332572..1333522 + 951 WP_003245086.1 ring-cleaving dioxygenase -
RP72_RS23235 1333623..1333769 + 147 WP_003244977.1 hypothetical protein -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(3-238)

Antitoxin

(1-56)


Sequences


Toxin        


Download         Length: 249 a.a.        Molecular weight: 29061.50 Da        Isoelectric Point: 4.6191

>T51991 WP_003244695.1 NZ_CP010314:c1328850-1328104 [Bacillus subtilis subsp. subtilis]
MVLFFQIMVWCIVAGLGLYVYATWRFEAKVKEKMSAIRKTWYLLFVLGAMVYWTYEPTSLFTHWERYLIVAVSFALIDAF
IFLSAYVKKLAGSELETDTREILEENNEMLHMYLNRLKTYQYLLKNEPIHVYYGSIDAYAEGIDKLLKTYADKMNLTASL
CHYSTQADKDRLTEHMDDPADVQTRLDRKDVYYDQYGKVVLIPFTIETQNYVIKLTSDSIVTEFDYLLFTSLTSIYDLVL
PIEEEGEG

Download         Length: 747 bp

>T51991 NZ_CP010314:c1328850-1328104 [Bacillus subtilis subsp. subtilis]
ATGGTTTTATTCTTTCAGATCATGGTCTGGTGCATCGTGGCCGGACTGGGGTTATACGTGTATGCCACGTGGCGTTTCGA
AGCGAAGGTCAAAGAAAAAATGTCCGCCATTCGGAAAACTTGGTATTTGCTGTTTGTTCTGGGCGCTATGGTATACTGGA
CATATGAGCCCACTTCCCTATTTACCCACTGGGAACGGTATCTCATTGTCGCAGTCAGTTTTGCTTTGATTGATGCTTTT
ATCTTCTTAAGTGCATATGTCAAAAAACTGGCCGGCAGCGAGCTTGAAACAGACACAAGAGAAATTCTTGAAGAAAACAA
CGAAATGCTCCACATGTATCTCAATCGGCTGAAAACATACCAATACCTATTGAAAAACGAACCGATCCATGTTTATTATG
GAAGTATAGATGCTTATGCTGAAGGTATTGATAAGCTGCTGAAAACCTATGCTGATAAAATGAACTTAACGGCTTCTCTT
TGCCACTATTCGACACAGGCTGATAAAGACCGGTTAACCGAGCATATGGATGATCCGGCAGATGTACAAACACGGCTCGA
TCGAAAGGATGTTTATTACGACCAATACGGAAAAGTGGTTCTCATCCCTTTTACCATCGAGACACAGAACTATGTCATCA
AGCTGACGTCTGACAGCATTGTCACGGAATTTGATTATTTGCTATTTACGTCATTAACGAGCATATATGATTTGGTGCTG
CCAATTGAGGAGGAAGGTGAAGGATAA

Antitoxin


Download         Length: 57 a.a.        Molecular weight: 6654.72 Da        Isoelectric Point: 11.0203

>AT51991 WP_003232646.1 NZ_CP010314:c1328104-1327934 [Bacillus subtilis subsp. subtilis]
MERAFQNRCEPRAAKPFKILKKRSTTSVASYQVSPHTARIFKENERLIDEYKRKKA

Download         Length: 171 bp

>AT51991 NZ_CP010314:c1328104-1327934 [Bacillus subtilis subsp. subtilis]
ATGGAACGTGCGTTTCAAAACAGATGCGAGCCCAGAGCGGCGAAGCCGTTTAAAATCCTGAAAAAACGTTCAACCACCAG
TGTCGCAAGCTATCAAGTCAGTCCGCATACAGCAAGAATCTTCAAAGAAAACGAACGGCTGATTGACGAGTATAAACGAA
AAAAAGCATGA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
PDB 3O6Q


Antitoxin

Source ID Structure
AlphaFold DB G4EXD8

References