Detailed information of TA system TA051989

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PemK-MazE
• Location: 7680992..7681563
• Replicon: chromosome
• Accession: NZ_CP010313
• Organism: Bradyrhizobium japonicum strain E109

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: A0A845IZB5
• Locus tag: RN69_RS35785
• Protein ID: WP_014497460.1
• Coordinates: 7681210..7681563 (+)
• Length: 118 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: RN69_RS43955
• Protein ID: WP_080583987.1
• Coordinates: 7680992..7681213 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RN69_RS35775	7676338..7679475	-	3138	WP_014497458.1	efflux RND transporter permease subunit	-
RN69_RS35780	7679709..7680815	-	1107	WP_028170414.1	efflux RND transporter periplasmic adaptor subunit	-
RN69_RS43955	7680992..7681213	+	222	WP_080583987.1	antitoxin MazE family protein	Antitoxin
RN69_RS35785	7681210..7681563	+	354	WP_014497460.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
RN69_RS35790	7681629..7682960	-	1332	WP_014497461.1	MFS transporter	-
RN69_RS35795	7683041..7683469	+	429	WP_014497462.1	DUF2809 domain-containing protein	-
RN69_RS35800	7683488..7684651	-	1164	WP_014497463.1	winged helix-turn-helix domain-containing protein	-
RN69_RS35805	7684766..7685011	-	246	WP_014497464.1	hypothetical protein	-
RN69_RS35810	7685110..7685346	-	237	WP_014497465.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (2-104)

Antitoxin

• Antitoxin MazE-like (12-73)

Sequences

Toxin

Protein

• Length: 118 a.a.
• Molecular weight: 12687.49 Da
• Isoelectric Point: 6.3275

>T51989 WP_014497460.1 NZ_CP010313:7681210-7681563 [Bradyrhizobium japonicum]
MKRGEIWTVAGGKDYAGKPRPVVIVQDDSFDATNSITVCAFTTDETEAPLFRLKVEPNDNNGLRVPSRLMVDKITTVPKA
KVGTLVGRLDDDDLLRLNRAVLVFLGLAGSQRSASAD

Nucleotide

• Length: 354 bp

>T51989 NZ_CP010313:7681210-7681563 [Bradyrhizobium japonicum]
ATGAAGCGAGGTGAGATCTGGACGGTGGCAGGCGGCAAGGACTATGCGGGCAAGCCGCGCCCCGTCGTCATCGTGCAGGA
CGACAGCTTCGACGCCACGAACTCGATCACTGTCTGCGCTTTCACAACCGACGAAACCGAGGCGCCGCTTTTCCGGCTCA
AGGTCGAGCCAAACGACAACAACGGCCTTCGAGTGCCGTCCCGGCTGATGGTCGATAAGATTACCACGGTGCCGAAAGCC
AAGGTGGGAACGCTAGTCGGTCGCCTAGACGATGACGACCTCTTGCGTCTCAACCGCGCTGTGCTGGTGTTCCTTGGACT
GGCTGGTTCGCAGAGAAGCGCCAGCGCAGATTAG

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8248.25 Da
• Isoelectric Point: 10.4527

>AT51989 WP_080583987.1 NZ_CP010313:7680992-7681213 [Bradyrhizobium japonicum]
MSPSKPKPSRLKVQAHRDRLRAQGLRPIQIWVPDIRSPSFRSEAHRQSLAVATSVHASEDQGFIDAVSDWTDE

Nucleotide

• Length: 222 bp

>AT51989 NZ_CP010313:7680992-7681213 [Bradyrhizobium japonicum]
ATGAGCCCTTCAAAGCCCAAGCCGTCTCGTCTGAAGGTGCAGGCCCACCGTGATCGATTGCGCGCACAAGGCCTGCGGCC
GATTCAGATTTGGGTGCCGGATATTCGCTCTCCGTCCTTTCGATCGGAAGCCCATCGCCAATCGCTTGCGGTCGCGACCA
GTGTACATGCATCCGAAGACCAGGGCTTCATCGACGCCGTATCCGATTGGACGGATGAATGA

Structures

Toxin

• AlphaFold DB: A0A845IZB5