Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 5198775..5199272 Replicon chromosome
Accession NZ_CP010304
Organism Escherichia coli O157:H7 str. SS52

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag SS52_RS26910 Protein ID WP_001173343.1
Coordinates 5199099..5199272 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag SS52_RS26905 Protein ID WP_000398619.1
Coordinates 5198775..5199071 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
SS52_RS26880 5195506..5196225 - 720 WP_000611288.1 two-component system response regulator DcuR -
SS52_RS26885 5196222..5197853 - 1632 WP_001216477.1 sensor histidine kinase -
SS52_RS26890 5198034..5198264 + 231 WP_000371704.1 (4Fe-4S)-binding protein -
SS52_RS26895 5198276..5198548 + 273 WP_000405647.1 N-acetyltransferase -
SS52_RS26905 5198775..5199071 + 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
SS52_RS26910 5199099..5199272 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
SS52_RS26915 5199391..5200908 - 1518 WP_001295074.1 lysine--tRNA ligase -
SS52_RS26920 5201145..5202602 - 1458 WP_000856824.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T51969 WP_001173343.1 NZ_CP010304:5199099-5199272 [Escherichia coli O157:H7 str. SS52]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T51969 NZ_CP010304:5199099-5199272 [Escherichia coli O157:H7 str. SS52]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT51969 WP_000398619.1 NZ_CP010304:5198775-5199071 [Escherichia coli O157:H7 str. SS52]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT51969 NZ_CP010304:5198775-5199071 [Escherichia coli O157:H7 str. SS52]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCTTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GACTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References