Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 4379072..4379703 Replicon chromosome
Accession NZ_CP010304
Organism Escherichia coli O157:H7 str. SS52

Toxin (Protein)


Gene name hicA Uniprot ID U9Y2A7
Locus tag SS52_RS22920 Protein ID WP_001259385.1
Coordinates 4379428..4379703 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag SS52_RS22915 Protein ID WP_000593555.1
Coordinates 4379072..4379431 (-) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
SS52_RS22885 4374184..4374657 + 474 WP_001161553.1 PTS sugar transporter subunit IIA -
SS52_RS22890 4374654..4374935 + 282 WP_000084025.1 PTS sugar transporter subunit IIB -
SS52_RS22895 4375012..4376370 + 1359 WP_001302220.1 PTS galactitol transporter subunit IIC -
SS52_RS22900 4376363..4377871 + 1509 WP_001301773.1 carbohydrate kinase -
SS52_RS22905 4377861..4378130 + 270 WP_000933060.1 HPr family phosphocarrier protein -
SS52_RS22910 4378162..4379022 + 861 WP_001131788.1 class II aldolase -
SS52_RS22915 4379072..4379431 - 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
SS52_RS22920 4379428..4379703 - 276 WP_001259385.1 type II toxin-antitoxin system HicA family toxin Toxin
SS52_RS22925 4379776..4380900 - 1125 WP_001301659.1 ABC transporter permease -
SS52_RS22930 4380900..4383635 - 2736 WP_000149110.1 ribosome-associated ATPase/putative transporter RbbA -
SS52_RS22935 4383632..4384699 - 1068 WP_000361458.1 HlyD family secretion protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10199.94 Da        Isoelectric Point: 10.7076

>T51966 WP_001259385.1 NZ_CP010304:c4379703-4379428 [Escherichia coli O157:H7 str. SS52]
MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T51966 NZ_CP010304:c4379703-4379428 [Escherichia coli O157:H7 str. SS52]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATATTTAAAACTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGTAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT51966 WP_000593555.1 NZ_CP010304:c4379431-4379072 [Escherichia coli O157:H7 str. SS52]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT51966 NZ_CP010304:c4379431-4379072 [Escherichia coli O157:H7 str. SS52]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCTGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TTW8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References