Detailed information of TA system TA051950

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): paaR-paaA-parE/-
• Location: 2115037..2115508
• Replicon: chromosome
• Accession: NZ_CP010304
• Organism: Escherichia coli O157:H7 str. SS52

Toxin (Protein)

• Gene name: parE_1
• Uniprot ID: A0A0D7C2L1
• Locus tag: SS52_RS10865
• Protein ID: WP_001303511.1
• Coordinates: 2115230..2115508 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: paaA
• Uniprot ID: Q8XAD5
• Locus tag: SS52_RS10860
• Protein ID: WP_001302048.1
• Coordinates: 2115037..2115228 (+)
• Length: 64 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SS52_RS10815	2110244..2110828	-	585	WP_000206793.1	DUF551 domain-containing protein	-
SS52_RS10820	2110885..2111280	-	396	WP_001118159.1	DUF977 family protein	-
SS52_RS10825	2111296..2112065	-	770	Protein_2046	DUF1627 domain-containing protein	-
SS52_RS10835	2112091..2112831	-	741	WP_000788938.1	ATP-binding protein	-
SS52_RS10840	2112838..2113800	-	963	WP_000095669.1	helix-turn-helix domain-containing protein	-
SS52_RS10845	2113823..2114248	-	426	WP_000693943.1	Rha family transcriptional regulator	-
SS52_RS10850	2114245..2114547	-	303	WP_000172738.1	transcriptional regulator	-
SS52_RS31840	2114645..2115016	+	372	WP_001169686.1	hypothetical protein	-
SS52_RS10860	2115037..2115228	+	192	WP_001302048.1	hypothetical protein	Antitoxin
SS52_RS10865	2115230..2115508	+	279	WP_001303511.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
SS52_RS10875	2115861..2116160	+	300	WP_001240334.1	hypothetical protein	-
SS52_RS10880	2116232..2116450	+	219	WP_001171930.1	hypothetical protein	-
SS52_RS30020	2117019..2117207	+	189	WP_000449192.1	cell division inhibition protein DicB	-
SS52_RS10885	2117204..2117395	+	192	WP_001090200.1	DUF1482 family protein	-
SS52_RS10890	2117488..2118573	+	1086	Protein_2058	exonuclease	-
SS52_RS10900	2118624..2119837	+	1214	WP_162829202.1	IS3 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	nleG7'	2014952..2136829	121877

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-91)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10661.25 Da
• Isoelectric Point: 5.5647

>T51950 WP_001303511.1 NZ_CP010304:2115230-2115508 [Escherichia coli O157:H7 str. SS52]
MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLSEHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSVE
VVNVIHARRQFP

Nucleotide

• Length: 279 bp

>T51950 NZ_CP010304:2115230-2115508 [Escherichia coli O157:H7 str. SS52]
ATGTTACCCGTGTTATGGCTTGAAAGCGCAGATACCGACCTAGATGATATAACTAGTTATATTGCTCGTTTCGACATAGA
TGCGGCTGAACGCTTATGGCAGCGATTAAGGGGTTGTGTGCTGCCGTTATCCGAACATCCGTATTTATACCCACCAAGCG
ACAGAGTACCTGGCTTGCGTGAGATTGTAGCCCACCCTAACTATATAATTCTATACCGCGTAACAACATCAAGCGTTGAA
GTAGTAAACGTGATCCACGCAAGACGCCAGTTTCCCTAA

Antitoxin

Protein

• Length: 64 a.a.
• Molecular weight: 7463.47 Da
• Isoelectric Point: 8.6828

>AT51950 WP_001302048.1 NZ_CP010304:2115037-2115228 [Escherichia coli O157:H7 str. SS52]
MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDEVERRMAERFAKMRKERSKQ

Nucleotide

• Length: 192 bp

>AT51950 NZ_CP010304:2115037-2115228 [Escherichia coli O157:H7 str. SS52]
ATGAATAGAGCCCTTTCACCAATGGTTTCTGAATTTGAAACCATTGAACAAGAAAACAGTTACAACGAATGGCTGCGTGC
GAAAGTAGCAACGAGCCTTGCAGATCCGCGCCCAGCAATTCCCCATGACGAAGTTGAGCGCAGAATGGCAGAACGCTTTG
CTAAGATGCGCAAGGAACGGAGCAAGCAGTAA

Structures

Toxin

• PDB: 5CZF
• PDB: 5CW7
• PDB: 5CZE

Antitoxin

• PDB: 5CZF
• PDB: 3ZBE
• PDB: 5CW7
• AlphaFold DB: A0A829CTP0