Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 3510668..3511310 Replicon chromosome
Accession NZ_CP010272
Organism Bacillus cereus strain FORC_010

Toxin (Protein)


Gene name pemK Uniprot ID R8I8B8
Locus tag FORC10_RS17480 Protein ID WP_000635965.1
Coordinates 3510668..3511018 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag FORC10_RS17485 Protein ID WP_000004570.1
Coordinates 3511023..3511310 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FORC10_RS17465 (FORC10_3341) 3507604..3508062 - 459 WP_000344237.1 SprT family protein -
FORC10_RS17470 3508258..3508374 + 117 WP_001143642.1 cortex morphogenetic protein CmpA -
FORC10_RS17475 (FORC10_3342) 3508432..3510600 - 2169 WP_048558646.1 Tex family protein -
FORC10_RS17480 (FORC10_3343) 3510668..3511018 - 351 WP_000635965.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
FORC10_RS17485 (FORC10_3344) 3511023..3511310 - 288 WP_000004570.1 antitoxin EndoAI Antitoxin
FORC10_RS17490 (FORC10_3345) 3511619..3512788 - 1170 WP_000390615.1 alanine racemase -
FORC10_RS17495 (FORC10_3346) 3512906..3513856 - 951 WP_002004297.1 outer membrane lipoprotein carrier protein LolA -
FORC10_RS17500 (FORC10_3347) 3514013..3514372 - 360 WP_000583415.1 holo-ACP synthase -
FORC10_RS17505 (FORC10_3348) 3514466..3515038 + 573 WP_000908523.1 rhomboid family intramembrane serine protease -
FORC10_RS17510 (FORC10_3349) 3515031..3515993 - 963 WP_000961166.1 UV DNA damage repair endonuclease UvsE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12948.04 Da        Isoelectric Point: 5.7168

>T51835 WP_000635965.1 NZ_CP010272:c3511018-3510668 [Bacillus cereus]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMSRVDEALQISLGLIDF

Download         Length: 351 bp

>T51835 NZ_CP010272:c3511018-3510668 [Bacillus cereus]
TTGATTGTAAAACGCGGCGACGTGTATTTTGCAGACCTTTCCCCAGTTGTTGGTTCTGAGCAAGGAGGCGTTCGTCCGGT
TCTTGTCATTCAAAATGACATCGGAAATCGTTTTAGTCCAACGGTGATTGTAGCGGCTATTACTGCACAGATTCAAAAAG
CGAAATTACCCACTCATGTGGAAATTGATGCGAAAAAGTATGGTTTTGAGAGAGATTCTGTTATTTTACTTGAGCAGATT
CGAACAATCGATAAGCAACGCTTAACGGACAAAATCACTCATTTGGATGAAGTGATGATGAGTCGTGTAGATGAAGCGTT
ACAAATTAGTTTAGGACTAATAGATTTTTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT51835 WP_000004570.1 NZ_CP010272:c3511310-3511023 [Bacillus cereus]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT51835 NZ_CP010272:c3511310-3511023 [Bacillus cereus]
GTGTCCGAATCAAGTGTAACTACTGAAATCGTGGTTCGGTTGCCAAAGCAAATGGTAACGGAATTGGACGGAATTGGAAA
ACAAGAGAATAAGAATCGCCATGAACTAATTTGCCAGGCAACACAATTGTTATTGCGTCAACATAAGACGAAGAAACGCT
ACCAACATGAATCAATGCGACGTGGGTACATTGAAATGGGAAAAATTAATCTTGGTATTGCATCTGAAGCTTTCTTAGCA
GAGTATGAAGCAGCTCATACAGTAGAACGCTTAGTTAGCGGGGGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A366G118

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References