Detailed information of TA system TA051781

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ataT-KacA/DUF1778(antitoxin)
• Location: 69812..70610
• Replicon: plasmid B
• Accession: NZ_CP010244
• Organism: Escherichia coli strain S56

Toxin (Protein)

• Gene name: ataT
• Uniprot ID: -
• Locus tag: RG73_RS26100
• Protein ID: WP_074014842.1
• Coordinates: 70089..70610 (+)
• Length: 174 a.a.

Antitoxin (Protein)

• Gene name: KacA
• Uniprot ID: A0A141BRA2
• Locus tag: RG73_RS26095
• Protein ID: WP_001711191.1
• Coordinates: 69812..70081 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RG73_RS26070	65239..66579	-	1341	WP_074014841.1	AAA family ATPase	-
RG73_RS26075	66640..67365	-	726	WP_032187666.1	hypothetical protein	-
RG73_RS26080	67648..68415	+	768	WP_001711193.1	hypothetical protein	-
RG73_RS26085	68468..68821	-	354	WP_161953766.1	hypothetical protein	-
RG73_RS26090	68827..69492	-	666	WP_023135658.1	AAA family ATPase	-
RG73_RS26095	69812..70081	+	270	WP_001711191.1	DUF1778 domain-containing protein	Antitoxin
RG73_RS26100	70089..70610	+	522	WP_074014842.1	GNAT family N-acetyltransferase	Toxin
RG73_RS26110	70779..71030	-	252	WP_023135660.1	hypothetical protein	-
RG73_RS26115	71033..71725	-	693	WP_024172707.1	membrane protein	-
RG73_RS26120	71739..72062	-	324	WP_074014843.1	hypothetical protein	-
RG73_RS26125	72196..72777	-	582	WP_074014844.1	tail fiber assembly protein	-
RG73_RS27770	72777..73733	-	957	Protein_79	tail fiber protein	-
RG73_RS27775	74190..74408	-	219	Protein_80	tail fiber protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..113250	113250

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (85-150)

Antitoxin

• Vibrio phage ICP1, Orf50 (8-86)

Sequences

Toxin

Protein

• Length: 174 a.a.
• Molecular weight: 19393.20 Da
• Isoelectric Point: 8.2462

>T51781 WP_074014842.1 NZ_CP010244:70089-70610 [Escherichia coli]
VSNTTIEIFSGEKDYDLNGFDCGEESLNAFLTNHLKRQHEGKILRAYVLCTKEEIPKVLGYYTLSGSCFEKETLPSKSQQ
KKVPYRNVPSITLGRLALDKSLQGQGFGSMLVTHAMRVVYNASLAVGIHGLFVEALNDKARAFYKSLGFIQLVGNNDRSL
FYPTKSIEKLFEE

Nucleotide

• Length: 522 bp

>T51781 NZ_CP010244:70089-70610 [Escherichia coli]
GTGAGCAATACGACGATCGAGATTTTCTCTGGAGAGAAAGATTATGATCTAAACGGTTTTGATTGCGGCGAAGAGTCACT
AAACGCATTTTTGACCAACCACTTAAAAAGACAGCATGAAGGAAAAATTCTTCGCGCTTATGTGCTTTGCACTAAAGAAG
AAATACCAAAGGTATTAGGTTATTACACTTTGTCAGGCAGCTGCTTTGAGAAAGAAACCTTACCTTCAAAGAGTCAGCAA
AAGAAAGTGCCTTATCGGAATGTCCCAAGTATCACTTTAGGCAGACTGGCTTTGGACAAATCTCTTCAAGGTCAGGGGTT
TGGCTCAATGCTTGTAACTCACGCAATGCGTGTTGTGTACAATGCATCTCTTGCGGTAGGCATTCATGGGCTTTTCGTTG
AGGCGTTGAACGATAAAGCTAGGGCGTTTTATAAAAGTTTGGGCTTTATCCAGCTGGTTGGTAACAACGATCGTTCTTTG
TTCTATCCAACAAAATCTATCGAAAAATTGTTCGAAGAATAA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10054.53 Da
• Isoelectric Point: 8.7353

>AT51781 WP_001711191.1 NZ_CP010244:69812-70081 [Escherichia coli]
MSALKKQRIDLRLTDDDKSIIEEAAAMSNQSITQFMVSSASERAVKVIEQHRRLVLSEESWNLVMDAISNPPAPNDKLKR
AANRLKSME

Nucleotide

• Length: 270 bp

>AT51781 NZ_CP010244:69812-70081 [Escherichia coli]
ATGTCCGCACTGAAAAAGCAGCGCATCGATTTGAGATTAACCGATGACGATAAAAGCATCATCGAGGAAGCTGCCGCAAT
GTCTAACCAGAGCATTACCCAGTTCATGGTTAGCAGTGCATCCGAACGTGCCGTGAAGGTGATAGAGCAACACCGTAGAC
TGGTTCTAAGTGAAGAGTCCTGGAATCTGGTTATGGATGCTATAAGCAATCCTCCGGCACCGAACGACAAGCTGAAACGA
GCTGCCAATCGTCTGAAAAGCATGGAGTAA

Structures

Antitoxin

• AlphaFold DB: A0A141BRA2