Detailed information of TA system TA051780

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-RelB
• Location: 63235..63833
• Replicon: plasmid B
• Accession: NZ_CP010244
• Organism: Escherichia coli strain S56

Toxin (Protein)

• Gene name: doc
• Uniprot ID: -
• Locus tag: RG73_RS26060
• Protein ID: WP_032187669.1
• Coordinates: 63456..63833 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: L4J1C0
• Locus tag: RG73_RS26055
• Protein ID: WP_001603498.1
• Coordinates: 63235..63456 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RG73_RS26035	58241..58789	+	549	WP_074014839.1	fimbrial protein YehD	-
RG73_RS26040	58845..59525	+	681	WP_032187672.1	fimbrial assembly chaperone	-
RG73_RS26045	59543..62029	+	2487	WP_074014840.1	fimbria/pilus outer membrane usher protein	-
RG73_RS26050	62040..63050	+	1011	WP_032187670.1	fimbrial protein	-
RG73_RS26055	63235..63456	+	222	WP_001603498.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
RG73_RS26060	63456..63833	+	378	WP_032187669.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
RG73_RS26065	63967..65082	-	1116	WP_024144822.1	hypothetical protein	-
RG73_RS26070	65239..66579	-	1341	WP_074014841.1	AAA family ATPase	-
RG73_RS26075	66640..67365	-	726	WP_032187666.1	hypothetical protein	-
RG73_RS26080	67648..68415	+	768	WP_001711193.1	hypothetical protein	-
RG73_RS26085	68468..68821	-	354	WP_161953766.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..113250	113250

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (2-122)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-51)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13465.18 Da
• Isoelectric Point: 6.2257

>T51780 WP_032187669.1 NZ_CP010244:63456-63833 [Escherichia coli]
MRHISPEELTAIHDANISRYGGLPGMSDSGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVYDSPELAELTVGAATGEVSVSTVVATLRRLYGTA

Nucleotide

• Length: 378 bp

>T51780 NZ_CP010244:63456-63833 [Escherichia coli]
ATGAGGCATATATCACCGGAAGAGCTTACTGCAATTCATGATGCGAATATAAGCCGCTACGGCGGCCTGCCAGGCATGTC
AGATTCGGGAAGAGCTGAGGCTATTATCGGGAGAGTCCAGGCCAGAGTTGCATATGAAGAGATTACCGACCTTTTCGAAG
TCTCCGCAACTTACCTGGTGGCTACTGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
TTGTTATTTCTGCGCCGTAACGGTGTGCAGGTATATGATTCACCAGAACTGGCAGAGCTTACTGTGGGGGCTGCGACCGG
AGAGGTATCCGTATCTACTGTCGTTGCCACGTTACGTAGATTGTATGGTACGGCGTAG

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8190.24 Da
• Isoelectric Point: 6.3159

>AT51780 WP_001603498.1 NZ_CP010244:63235-63456 [Escherichia coli]
MQSINFRTARSNLTEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDTTNKKLVNR

Nucleotide

• Length: 222 bp

>AT51780 NZ_CP010244:63235-63456 [Escherichia coli]
ATGCAATCTATTAACTTCCGTACCGCACGCAGCAATCTTACCGAAGTGCTCAACAATGTTGAAGCCGGAGAAGAGGTTGA
AATCACCCGTAGAGGCCGTGAGCCAGCTGTAATTGTCAGCAAGGCCACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTCGCATCCCTGTTTGACACTCTGGACACCACCAATAAGAAACTGGTTAACCGATAA

Structures

Antitoxin

• AlphaFold DB: L4J1C0