Detailed information of TA system TA051778

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 4604918..4605543
• Replicon: chromosome
• Accession: NZ_CP010242
• Organism: Escherichia coli strain S56

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: RG73_RS24130
• Protein ID: WP_000911330.1
• Coordinates: 4604918..4605316 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: RG73_RS24135
• Protein ID: WP_000450524.1
• Coordinates: 4605316..4605543 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RG73_RS24110	4600796..4600996	+	201	WP_000383836.1	YpfN family protein	-
RG73_RS24115	4601106..4601804	-	699	WP_074014747.1	esterase	-
RG73_RS24120	4601878..4603893	-	2016	WP_000829292.1	tRNA cytosine(34) acetyltransferase TmcA	-
RG73_RS24125	4603908..4604771	-	864	WP_001267498.1	neutral zinc metallopeptidase	-
RG73_RS24130	4604918..4605316	-	399	WP_000911330.1	tRNA(fMet)-specific endonuclease VapC	Toxin
RG73_RS24135	4605316..4605543	-	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
RG73_RS24140	4605698..4606411	-	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
RG73_RS24150	4606624..4607658	-	1035	WP_001297320.1	outer membrane protein assembly factor BamC	-
RG73_RS24155	4607675..4608553	-	879	WP_001295469.1	4-hydroxy-tetrahydrodipicolinate synthase	-
RG73_RS24160	4608699..4609271	+	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
RG73_RS24165	4609271..4609741	+	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T51778 WP_000911330.1 NZ_CP010242:c4605316-4604918 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T51778 NZ_CP010242:c4605316-4604918 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT51778 WP_000450524.1 NZ_CP010242:c4605543-4605316 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT51778 NZ_CP010242:c4605543-4605316 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33