Detailed information of TA system TA051761

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 913202..913833
• Replicon: chromosome
• Accession: NZ_CP010242
• Organism: Escherichia coli strain S56

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: F4NNK6
• Locus tag: RG73_RS04900
• Protein ID: WP_001259388.1
• Coordinates: 913558..913833 (-)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A8S7TCM0
• Locus tag: RG73_RS04895
• Protein ID: WP_000593556.1
• Coordinates: 913202..913561 (-)
• Length: 120 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RG73_RS04870	909334..910278	+	945	WP_074014562.1	nickel ABC transporter permease subunit NikB	-
RG73_RS04875	910275..911108	+	834	WP_001008963.1	nickel ABC transporter permease subunit NikC	-
RG73_RS04880	911108..911872	+	765	WP_001136236.1	nickel import ATP-binding protein NikD	-
RG73_RS04885	911869..912675	+	807	WP_000173666.1	nickel import ATP-binding protein NikE	-
RG73_RS04890	912681..913082	+	402	WP_001190062.1	nickel-responsive transcriptional regulator NikR	-
RG73_RS04895	913202..913561	-	360	WP_000593556.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
RG73_RS04900	913558..913833	-	276	WP_001259388.1	type II toxin-antitoxin system HicA family toxin	Toxin
RG73_RS04905	913906..915030	-	1125	WP_001314210.1	ABC transporter permease	-
RG73_RS04910	915030..917765	-	2736	WP_074014563.1	ribosome-associated ATPase/putative transporter RbbA	-
RG73_RS04915	917762..918829	-	1068	WP_000361485.1	HlyD family secretion protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (30-80)

Antitoxin

• Uncharacterised protein family HicB (60-112)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10151.89 Da
• Isoelectric Point: 10.7076

>T51761 WP_001259388.1 NZ_CP010242:c913833-913558 [Escherichia coli]
MRTKVQALRKKQKNTLDQIVKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Nucleotide

• Length: 276 bp

>T51761 NZ_CP010242:c913833-913558 [Escherichia coli]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATAGTTAAAACTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGCAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin

Protein

• Length: 120 a.a.
• Molecular weight: 13388.25 Da
• Isoelectric Point: 5.1987

>AT51761 WP_000593556.1 NZ_CP010242:c913561-913202 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHV

Nucleotide

• Length: 360 bp

>AT51761 NZ_CP010242:c913561-913202 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCAGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATGTATAA

Structures

Toxin

• AlphaFold DB: A0A0A0FFA2