Detailed information of TA system TA051753

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 41062..41621
• Replicon: plasmid A
• Accession: NZ_CP010241
• Organism: Escherichia coli strain C7

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: RG29_RS28865
• Protein ID: WP_000421253.1
• Coordinates: 41346..41621 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: RG29_RS28860
• Protein ID: WP_024182491.1
• Coordinates: 41062..41346 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
-	36645..36706	-	62	NuclAT_1	-	-
-	36645..36706	-	62	NuclAT_1	-	-
-	36645..36706	-	62	NuclAT_1	-	-
-	36645..36706	-	62	NuclAT_1	-	-
RG29_RS28815	36693..36899	+	207	WP_032232098.1	type I toxin-antitoxin system Hok family toxin	-
RG29_RS28820	37221..37523	+	303	WP_001346256.1	DUF1778 domain-containing protein	-
RG29_RS28825	37514..37996	+	483	WP_000405244.1	GNAT family N-acetyltransferase	-
RG29_RS28830	38305..38562	+	258	WP_000083826.1	replication regulatory protein RepA	-
RG29_RS28840	38797..38871	+	75	WP_032164225.1	RepA leader peptide Tap	-
RG29_RS28845	38864..39346	+	483	WP_000247130.1	hypothetical protein	-
RG29_RS28850	39339..40196	+	858	WP_000774299.1	incFII family plasmid replication initiator RepA	-
RG29_RS28860	41062..41346	+	285	WP_024182491.1	ribbon-helix-helix protein, CopG family	Antitoxin
RG29_RS28865	41346..41621	+	276	WP_000421253.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
RG29_RS28870	41746..42498	+	753	Protein_42	DUF4158 domain-containing protein	-
RG29_RS28875	42495..42845	-	351	Protein_43	transposase family protein	-
RG29_RS28880	42947..43314	-	368	Protein_44	transposase	-
RG29_RS28885	43419..43619	-	201	WP_024182490.1	hypothetical protein	-
RG29_RS28905	44336..45033	+	698	Protein_46	IS1-like element IS1A family transposase	-
RG29_RS28910	45201..45584	-	384	WP_001063020.1	relaxosome protein TraM	-
RG29_RS28920	45936..46526	+	591	WP_001375132.1	transglycosylase SLT domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	faeC / faeD / faeE / faeF / faeH	1..62376	62376

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (14-51)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10755.41 Da
• Isoelectric Point: 9.5302

>T51753 WP_000421253.1 NZ_CP010241:41346-41621 [Escherichia coli]
MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPLILLTHPRMGEQLFQFDPREVRRFFAGEYEIRYELNGQTIY
VLRLWHTQENR

Nucleotide

• Length: 276 bp

>T51753 NZ_CP010241:41346-41621 [Escherichia coli]
ATGGAACTGAAGTGGACCAGTAAGGCGCTTTCTGATTTGGCGCGGTTATATGATTTTCTGGTACTGGCCAGTAAACCTGC
TGCCGCCAGAACGGTGCAGTCCCTGACACAGGCACCGCTCATTCTGTTAACTCATCCACGTATGGGAGAACAGTTGTTTC
AGTTTGACCCCAGGGAGGTCAGACGGTTTTTTGCTGGCGAGTACGAAATCCGTTACGAACTTAATGGCCAGACTATTTAT
GTATTGCGGCTGTGGCACACACAAGAAAACAGGTAG

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10497.98 Da
• Isoelectric Point: 6.7244

>AT51753 WP_024182491.1 NZ_CP010241:41062-41346 [Escherichia coli]
MRMKNNSAQATKVITAHVPLLMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
ADSLGTDHPLPVPR

Nucleotide

• Length: 285 bp

>AT51753 NZ_CP010241:41062-41346 [Escherichia coli]
ATGCGAATGAAAAACAATAGTGCACAAGCAACAAAAGTAATTACCGCACATGTGCCATTACTTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGGCTGGAACGCTCTCGGGGGTGGGTTATTAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATCGATCATCAGGCTGTACAGGCCTGG
GCGGACAGCCTCGGTACTGACCATCCGTTACCGGTGCCACGCTGA