Detailed information of TA system TA051752

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 37221..37996
• Replicon: plasmid A
• Accession: NZ_CP010241
• Organism: Escherichia coli strain C7

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: A0A8D9PPA8
• Locus tag: RG29_RS28825
• Protein ID: WP_000405244.1
• Coordinates: 37514..37996 (+)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: -
• Locus tag: RG29_RS28820
• Protein ID: WP_001346256.1
• Coordinates: 37221..37523 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RG29_RS28795	34356..35102	+	747	WP_000205747.1	conjugal transfer pilus acetylase TraX	-
RG29_RS28800	35157..35717	+	561	WP_024244031.1	fertility inhibition protein FinO	-
RG29_RS28805	35849..36061	+	213	WP_032323685.1	hypothetical protein	-
-	36645..36706	-	62	NuclAT_1	-	-
-	36645..36706	-	62	NuclAT_1	-	-
-	36645..36706	-	62	NuclAT_1	-	-
-	36645..36706	-	62	NuclAT_1	-	-
RG29_RS28815	36693..36899	+	207	WP_032232098.1	type I toxin-antitoxin system Hok family toxin	-
RG29_RS28820	37221..37523	+	303	WP_001346256.1	DUF1778 domain-containing protein	Antitoxin
RG29_RS28825	37514..37996	+	483	WP_000405244.1	GNAT family N-acetyltransferase	Toxin
RG29_RS28830	38305..38562	+	258	WP_000083826.1	replication regulatory protein RepA	-
RG29_RS28840	38797..38871	+	75	WP_032164225.1	RepA leader peptide Tap	-
RG29_RS28845	38864..39346	+	483	WP_000247130.1	hypothetical protein	-
RG29_RS28850	39339..40196	+	858	WP_000774299.1	incFII family plasmid replication initiator RepA	-
RG29_RS28860	41062..41346	+	285	WP_024182491.1	ribbon-helix-helix protein, CopG family	-
RG29_RS28865	41346..41621	+	276	WP_000421253.1	type II toxin-antitoxin system RelE/ParE family toxin	-
RG29_RS28870	41746..42498	+	753	Protein_42	DUF4158 domain-containing protein	-
RG29_RS28875	42495..42845	-	351	Protein_43	transposase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	faeC / faeD / faeE / faeF / faeH	1..62376	62376

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (30-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (15-99)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17659.41 Da
• Isoelectric Point: 7.1036

>T51752 WP_000405244.1 NZ_CP010241:37514-37996 [Escherichia coli]
MEINVTAPALLTDEHILQPFDCGNEVLSNWLHGRAMKNQMLNASRTFVICLEGTLRVVGYYSLATGSVTHAELGRSLRHN
MPNPVPVVLLGRLAVDVCTQGHGFGKWLLSDAIHRVVNLADQVGIKAVMVHAIDDDARAFYERFGFVQSVVAPNTLFYKV

Nucleotide

• Length: 483 bp

>T51752 NZ_CP010241:37514-37996 [Escherichia coli]
ATGGAAATAAATGTCACCGCACCGGCATTACTGACGGATGAGCATATACTTCAGCCATTTGATTGTGGGAATGAAGTGTT
GAGTAACTGGTTACATGGTCGCGCTATGAAAAACCAGATGCTCAATGCTTCCCGCACGTTCGTCATTTGCCTGGAGGGTA
CTTTACGTGTTGTGGGGTACTACTCATTAGCTACCGGTTCCGTCACTCATGCCGAACTTGGGCGCAGTTTGCGGCATAAC
ATGCCCAATCCTGTTCCTGTTGTTCTGTTAGGGCGTCTGGCTGTTGATGTTTGTACTCAGGGGCATGGTTTTGGTAAATG
GCTGCTGAGTGATGCCATTCATCGGGTTGTCAATCTGGCTGATCAGGTTGGTATTAAGGCTGTTATGGTTCATGCAATTG
ATGATGATGCCAGAGCGTTTTATGAGCGTTTTGGTTTTGTCCAGTCAGTTGTGGCACCAAATACCTTGTTTTATAAAGTC
TGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11602.19 Da
• Isoelectric Point: 4.4569

>AT51752 WP_001346256.1 NZ_CP010241:37221-37523 [Escherichia coli]
MEVVMPTTASIRKTPRENQINIRATDEERAVIDYAASLVNKNRTDFIIELAYQEAKNIILDQRLFVLDDECYDSFITQLE
APVQNAEGRERLMAVKPEWK

Nucleotide

• Length: 303 bp

>AT51752 NZ_CP010241:37221-37523 [Escherichia coli]
ATGGAGGTTGTTATGCCTACTACTGCAAGCATCCGTAAAACACCGCGAGAAAATCAGATTAACATCCGGGCAACGGATGA
GGAACGAGCTGTTATTGATTATGCCGCTAGCCTGGTCAATAAGAACCGTACTGATTTCATCATTGAACTGGCTTACCAGG
AAGCTAAAAACATCATCCTGGATCAACGTTTATTTGTGCTGGATGATGAATGTTATGATTCATTTATTACGCAACTTGAA
GCTCCTGTGCAGAATGCTGAAGGGCGTGAACGTCTGATGGCGGTAAAGCCTGAATGGAAATAA