Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) paaR-paaA-parE/-
Location 3066534..3067015 Replicon chromosome
Accession NZ_CP010240
Organism Escherichia coli strain C7

Toxin (Protein)


Gene name parE_1 Uniprot ID A0A0D7YS45
Locus tag RG29_RS16035 Protein ID WP_014966210.1
Coordinates 3066534..3066824 (-) Length 97 a.a.

Antitoxin (Protein)


Gene name paaA Uniprot ID A0A0D7Y007
Locus tag RG29_RS16040 Protein ID WP_000100896.1
Coordinates 3066824..3067015 (-) Length 64 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RG29_RS15995 3061891..3062160 - 270 WP_000003742.1 excisionase -
RG29_RS16000 3062222..3064693 - 2472 WP_000048520.1 exonuclease -
RG29_RS16005 3064786..3064977 - 192 WP_001090200.1 DUF1482 family protein -
RG29_RS16010 3064974..3065162 - 189 WP_000449192.1 cell division inhibition protein DicB -
RG29_RS16015 3065563..3065727 + 165 WP_001340038.1 hypothetical protein -
RG29_RS16020 3065731..3065949 - 219 WP_001171974.1 hypothetical protein -
RG29_RS16030 3066109..3066264 - 156 WP_000379575.1 DUF1391 family protein -
RG29_RS16035 3066534..3066824 - 291 WP_014966210.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
RG29_RS16040 3066824..3067015 - 192 WP_000100896.1 hypothetical protein Antitoxin
RG29_RS16045 3067033..3067533 - 501 WP_000823909.1 helix-turn-helix transcriptional regulator -
RG29_RS16050 3067641..3067916 + 276 WP_001048458.1 helix-turn-helix domain-containing protein -
RG29_RS16055 3067900..3068325 + 426 WP_000693918.1 Rha family transcriptional regulator -
RG29_RS29950 3068397..3069413 + 1017 WP_001262344.1 hypothetical protein -
RG29_RS29955 3069325..3069867 + 543 WP_072130322.1 replication protein -
RG29_RS16070 3069901..3070671 + 771 WP_000450649.1 DUF1627 domain-containing protein -
RG29_RS16075 3070687..3071109 + 423 WP_001151239.1 DUF977 family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - espW / espM2 3056536..3118456 61920
- flank IS/Tn - - 3071188..3072168 980


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-91)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 97 a.a.        Molecular weight: 11176.05 Da        Isoelectric Point: 9.9854

>T51727 WP_014966210.1 NZ_CP010240:c3066824-3066534 [Escherichia coli]
MLPILWLPSARDDLRQIVAYIAKENPPAARRLKIRIETSVLSLTEHPYLYPPSERVPSLREIVTHPNYIILYRVTASNIE
IVNVVHSRRQYPNKTC

Download         Length: 291 bp

>T51727 NZ_CP010240:c3066824-3066534 [Escherichia coli]
ATGCTGCCCATTTTATGGCTACCTTCTGCACGTGATGATTTACGTCAGATCGTAGCCTATATTGCTAAGGAAAACCCTCC
CGCTGCACGTAGACTAAAAATACGCATTGAAACATCAGTTTTGTCACTTACTGAACACCCTTATCTGTACCCACCGAGCG
AAAGAGTTCCAAGTCTTCGTGAGATAGTGACTCATCCTAACTACATAATACTTTACCGAGTAACAGCATCTAACATCGAG
ATCGTAAATGTAGTTCACTCACGAAGACAGTATCCAAACAAAACCTGTTAA

Antitoxin


Download         Length: 64 a.a.        Molecular weight: 7033.83 Da        Isoelectric Point: 4.0270

>AT51727 WP_000100896.1 NZ_CP010240:c3067015-3066824 [Escherichia coli]
MSTALSPIISEFETVEQENSYNEWLRTKVAASLSDPRPAIPHDEVMAEMENLIAQLAATNRSE

Download         Length: 192 bp

>AT51727 NZ_CP010240:c3067015-3066824 [Escherichia coli]
ATGAGTACAGCCCTTTCCCCGATAATTTCTGAATTTGAAACAGTCGAACAAGAAAACAGCTATAACGAATGGTTGCGAAC
CAAAGTGGCAGCAAGCCTCTCAGATCCCCGTCCTGCAATTCCACATGACGAAGTAATGGCTGAAATGGAAAACCTTATTG
CTCAATTAGCTGCAACGAACAGGAGTGAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0D7YS45

Antitoxin

Source ID Structure
AlphaFold DB A0A0D7Y007

References