Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 1951866..1952497 Replicon chromosome
Accession NZ_CP010240
Organism Escherichia coli strain C7

Toxin (Protein)


Gene name hicA Uniprot ID U9Y2A7
Locus tag RG29_RS10055 Protein ID WP_001259385.1
Coordinates 1951866..1952141 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID Q31VE4
Locus tag RG29_RS10060 Protein ID WP_000593554.1
Coordinates 1952138..1952497 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RG29_RS10040 1946870..1947937 + 1068 WP_000361467.1 HlyD family secretion protein -
RG29_RS10045 1947934..1950669 + 2736 WP_000149167.1 ribosome-associated ATPase/putative transporter RbbA -
RG29_RS10050 1950669..1951793 + 1125 WP_001216257.1 ABC transporter permease -
RG29_RS10055 1951866..1952141 + 276 WP_001259385.1 type II toxin-antitoxin system HicA family toxin Toxin
RG29_RS10060 1952138..1952497 + 360 WP_000593554.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
RG29_RS10065 1952547..1953407 - 861 Protein_1781 class II aldolase -
RG29_RS10070 1953439..1953708 - 270 WP_000933060.1 HPr family phosphocarrier protein -
RG29_RS10075 1953698..1955206 - 1509 WP_001298718.1 carbohydrate kinase -
RG29_RS10080 1955199..1956557 - 1359 WP_001298729.1 PTS galactitol transporter subunit IIC -
RG29_RS10085 1956634..1956915 - 282 WP_000084022.1 PTS sugar transporter subunit IIB -
RG29_RS10090 1956912..1957385 - 474 WP_001161553.1 PTS sugar transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 1944249..1952497 8248


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10199.94 Da        Isoelectric Point: 10.7076

>T51722 WP_001259385.1 NZ_CP010240:1951866-1952141 [Escherichia coli]
MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T51722 NZ_CP010240:1951866-1952141 [Escherichia coli]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATATTTAAAACTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGTAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13359.25 Da        Isoelectric Point: 4.8838

>AT51722 WP_000593554.1 NZ_CP010240:1952138-1952497 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLLEYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT51722 NZ_CP010240:1952138-1952497 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCAGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCTAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TTW8

Antitoxin

Source ID Structure
AlphaFold DB A0A6H2GM94

References