Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) ypjF-yeeU/CbtA-CbeA
Location 962911..963686 Replicon chromosome
Accession NZ_CP010240
Organism Escherichia coli strain C7

Toxin (Protein)


Gene name ypjF Uniprot ID A0A7V7JG91
Locus tag RG29_RS05035 Protein ID WP_001193488.1
Coordinates 962911..963282 (-) Length 124 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID A0A7V7JFU6
Locus tag RG29_RS05040 Protein ID WP_001059301.1
Coordinates 963321..963686 (-) Length 122 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RG29_RS05010 958869..959585 + 717 WP_001295734.1 N-acetylneuraminic acid outer membrane channel NanC -
RG29_RS05015 959605..960711 + 1107 WP_001298927.1 N-acetylneuraminate epimerase -
RG29_RS05020 960776..961756 + 981 WP_000991444.1 9-O-acetyl-N-acetylneuraminic acid deacetylase -
RG29_RS05035 962911..963282 - 372 WP_001193488.1 TA system toxin CbtA family protein Toxin
RG29_RS05040 963321..963686 - 366 WP_001059301.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
RG29_RS05045 963712..963933 - 222 WP_000691983.1 DUF987 domain-containing protein -
RG29_RS05050 963930..964472 - 543 WP_015740440.1 DNA repair protein RadC -
RG29_RS05055 964485..964928 - 444 WP_001096016.1 antirestriction protein -
RG29_RS05060 964959..965780 - 822 WP_001234409.1 DUF945 domain-containing protein -
RG29_RS05065 965900..966373 - 474 WP_001298943.1 hypothetical protein -
RG29_RS05070 966445..966897 - 453 WP_000734321.1 hypothetical protein -
RG29_RS05075 966933..967649 - 717 WP_000174910.1 WYL domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - fimE / fimB 955737..970427 14690


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(17-117)


Sequences

Toxin        


Download         Length: 124 a.a.        Molecular weight: 13832.94 Da        Isoelectric Point: 7.2419

>T51718 WP_001193488.1 NZ_CP010240:c963282-962911 [Escherichia coli]
MQTISSHPTRATQPCLSPVEIWQRLLTCLLSQHYGLTLNDTPFSNETTILEHIDAGVSLCDAVNFLVEKYELVRIDCNDC
SVMEKSPFITSIDILRARKASGLMKRNSHKTVTRVTAGRLQEL

Download         Length: 372 bp

>T51718 NZ_CP010240:c963282-962911 [Escherichia coli]
ATGCAAACTATATCATCACACCCGACACGGGCGACTCAGCCCTGCCTGTCACCCGTTGAAATCTGGCAGCGGTTACTGAC
TTGTCTGTTGTCGCAGCACTATGGTCTGACGTTGAATGACACACCGTTCAGTAATGAAACCACCATACTGGAACACATTG
ATGCTGGAGTATCGCTGTGCGATGCGGTGAACTTTCTTGTTGAAAAATATGAACTGGTAAGAATTGACTGTAATGATTGC
TCTGTCATGGAGAAGTCACCGTTCATCACCAGTATCGATATTCTACGTGCCCGGAAAGCCTCCGGTCTGATGAAACGCAA
CAGTCACAAAACTGTTACCAGGGTAACGGCAGGTCGACTTCAGGAGCTATAA

Antitoxin


Download         Length: 122 a.a.        Molecular weight: 13388.25 Da        Isoelectric Point: 5.9530

>AT51718 WP_001059301.1 NZ_CP010240:c963686-963321 [Escherichia coli]
MNNHSESGTKPENPACQQWGLKCAITPCFGARLVQEGNRLHFLSDRAGFSGAFAVDVAMRLDQAFPLMMKQLELMLTSGE
LNPRHPHCVTLYHNGLTCEADTLGSCGYVYIAIYPEQTEPQ

Download         Length: 366 bp

>AT51718 NZ_CP010240:c963686-963321 [Escherichia coli]
ATGAACAATCACTCAGAATCCGGTACAAAGCCGGAGAATCCCGCCTGCCAGCAGTGGGGGCTTAAATGTGCTATTACCCC
CTGTTTTGGTGCCCGCCTGGTACAGGAGGGCAACCGATTGCATTTTCTCTCTGACCGCGCAGGATTTAGCGGGGCATTTG
CCGTCGATGTTGCAATGCGCCTTGACCAGGCATTTCCGTTAATGATGAAGCAACTTGAGCTGATGCTGACCAGTGGAGAG
CTTAACCCTCGCCACCCACACTGCGTCACGCTTTACCACAATGGACTCACCTGTGAAGCCGATACACTAGGCTCCTGTGG
TTATGTCTACATTGCTATCTATCCTGAGCAAACTGAGCCGCAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7V7JG91

Antitoxin

Source ID Structure
AlphaFold DB A0A7V7JFU6

References