Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) ypjF-yeeU/CbtA-CbeA
Location 2596920..2597695 Replicon chromosome
Accession NZ_CP010238
Organism Escherichia coli strain S50

Toxin (Protein)


Gene name ypjF Uniprot ID A0A7V7JG91
Locus tag RG72_RS13600 Protein ID WP_001193488.1
Coordinates 2597324..2597695 (+) Length 124 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID A0A7V7JFU6
Locus tag RG72_RS13595 Protein ID WP_001059301.1
Coordinates 2596920..2597285 (+) Length 122 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RG72_RS13555 2592507..2592854 - 348 WP_000624622.1 IS66 family insertion sequence element accessory protein TnpB -
RG72_RS13560 2592854..2593531 - 678 WP_001299364.1 IS66 family insertion sequence hypothetical protein -
RG72_RS13565 2593709..2594161 + 453 WP_073569646.1 hypothetical protein -
RG72_RS13570 2594233..2594706 + 474 WP_001298943.1 hypothetical protein -
RG72_RS13575 2594826..2595647 + 822 WP_001234409.1 DUF945 domain-containing protein -
RG72_RS13580 2595678..2596121 + 444 WP_001096016.1 antirestriction protein -
RG72_RS13585 2596134..2596676 + 543 WP_015740440.1 DNA repair protein RadC -
RG72_RS13590 2596673..2596894 + 222 WP_000691983.1 DUF987 domain-containing protein -
RG72_RS13595 2596920..2597285 + 366 WP_001059301.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
RG72_RS13600 2597324..2597695 + 372 WP_001193488.1 TA system toxin CbtA family protein Toxin
RG72_RS13610 2598269..2599267 + 999 WP_001240355.1 membrane protein -
RG72_RS13615 2599378..2600250 - 873 WP_000168567.1 HNH endonuclease -
RG72_RS13620 2600332..2600454 + 123 WP_001338066.1 hypothetical protein -
RG72_RS13625 2600462..2601442 - 981 WP_001366032.1 9-O-acetyl-N-acetylneuraminic acid deacetylase -
RG72_RS13630 2601507..2602613 - 1107 WP_001044128.1 N-acetylneuraminate epimerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - fimB / fimE 2585631..2606480 20849


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(17-117)


Sequences

Toxin        


Download         Length: 124 a.a.        Molecular weight: 13832.94 Da        Isoelectric Point: 7.2419

>T51693 WP_001193488.1 NZ_CP010238:2597324-2597695 [Escherichia coli]
MQTISSHPTRATQPCLSPVEIWQRLLTCLLSQHYGLTLNDTPFSNETTILEHIDAGVSLCDAVNFLVEKYELVRIDCNDC
SVMEKSPFITSIDILRARKASGLMKRNSHKTVTRVTAGRLQEL

Download         Length: 372 bp

>T51693 NZ_CP010238:2597324-2597695 [Escherichia coli]
ATGCAAACTATATCATCACACCCGACACGGGCGACTCAGCCCTGCCTGTCACCCGTTGAAATCTGGCAGCGGTTACTGAC
TTGTCTGTTGTCGCAGCACTATGGTCTGACGTTGAATGACACACCGTTCAGTAATGAAACCACCATACTGGAACACATTG
ATGCTGGAGTATCGCTGTGCGATGCGGTGAACTTTCTTGTTGAAAAATATGAACTGGTAAGAATTGACTGTAATGATTGC
TCTGTCATGGAGAAGTCACCGTTCATCACCAGTATCGATATTCTACGTGCCCGGAAAGCCTCCGGTCTGATGAAACGCAA
CAGTCACAAAACTGTTACCAGGGTAACGGCAGGTCGACTTCAGGAGCTATAA

Antitoxin


Download         Length: 122 a.a.        Molecular weight: 13388.25 Da        Isoelectric Point: 5.9530

>AT51693 WP_001059301.1 NZ_CP010238:2596920-2597285 [Escherichia coli]
MNNHSESGTKPENPACQQWGLKCAITPCFGARLVQEGNRLHFLSDRAGFSGAFAVDVAMRLDQAFPLMMKQLELMLTSGE
LNPRHPHCVTLYHNGLTCEADTLGSCGYVYIAIYPEQTEPQ

Download         Length: 366 bp

>AT51693 NZ_CP010238:2596920-2597285 [Escherichia coli]
ATGAACAATCACTCAGAATCCGGTACAAAGCCGGAGAATCCCGCCTGCCAGCAGTGGGGGCTTAAATGTGCTATTACCCC
CTGTTTTGGTGCCCGCCTGGTACAGGAGGGCAACCGATTGCATTTTCTCTCTGACCGCGCAGGATTTAGCGGGGCATTTG
CCGTCGATGTTGCAATGCGCCTTGACCAGGCATTTCCGTTAATGATGAAGCAACTTGAGCTGATGCTGACCAGTGGAGAG
CTTAACCCTCGCCACCCACACTGCGTCACGCTTTACCACAATGGACTCACCTGTGAAGCCGATACACTAGGCTCCTGTGG
TTATGTCTACATTGCTATCTATCCTGAGCAAACTGAGCCGCAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7V7JG91

Antitoxin

Source ID Structure
AlphaFold DB A0A7V7JFU6

References