Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 1038683..1039337 Replicon chromosome
Accession NZ_CP010238
Organism Escherichia coli strain S50

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag RG72_RS05470 Protein ID WP_000244777.1
Coordinates 1038683..1039090 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag RG72_RS05475 Protein ID WP_000354046.1
Coordinates 1039071..1039337 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RG72_RS05450 1034640..1036373 - 1734 WP_000813215.1 single-stranded-DNA-specific exonuclease RecJ -
RG72_RS05455 1036379..1037089 - 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
RG72_RS05460 1037114..1038010 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
RG72_RS05465 1038122..1038643 + 522 WP_001055870.1 flavodoxin FldB -
RG72_RS05470 1038683..1039090 - 408 WP_000244777.1 toxin CptA Toxin
RG72_RS05475 1039071..1039337 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
RG72_RS05480 1039580..1040560 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
RG72_RS05485 1040756..1041415 - 660 WP_000250274.1 hemolysin III family protein -
RG72_RS05490 1041579..1041890 - 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
RG72_RS05495 1041935..1043368 + 1434 WP_001363803.1 6-phospho-beta-glucosidase BglA -
RG72_RS05500 1043425..1044168 - 744 WP_000951948.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T51687 WP_000244777.1 NZ_CP010238:c1039090-1038683 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T51687 NZ_CP010238:c1039090-1038683 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCTGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT51687 WP_000354046.1 NZ_CP010238:c1039337-1039071 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT51687 NZ_CP010238:c1039337-1039071 [Escherichia coli]
ATGGACATTAATAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References