Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 902251..902834 Replicon chromosome
Accession NZ_CP010238
Organism Escherichia coli strain S50

Toxin (Protein)


Gene name mazF Uniprot ID -
Locus tag RG72_RS04815 Protein ID WP_073569623.1
Coordinates 902251..902586 (-) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S1P3W5
Locus tag RG72_RS04820 Protein ID WP_000581937.1
Coordinates 902586..902834 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RG72_RS04795 897720..898082 + 363 WP_000034929.1 hypothetical protein -
RG72_RS04800 898138..899436 - 1299 WP_000036723.1 phosphopyruvate hydratase -
RG72_RS04805 899524..901161 - 1638 WP_000210878.1 CTP synthase (glutamine hydrolyzing) -
RG72_RS04810 901389..902180 - 792 WP_001071648.1 nucleoside triphosphate pyrophosphohydrolase -
RG72_RS04815 902251..902586 - 336 WP_073569623.1 endoribonuclease MazF Toxin
RG72_RS04820 902586..902834 - 249 WP_000581937.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
RG72_RS04825 902912..905146 - 2235 WP_000226828.1 GTP diphosphokinase -
RG72_RS04830 905194..906495 - 1302 WP_000046785.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12188.10 Da        Isoelectric Point: 8.4777

>T51686 WP_073569623.1 NZ_CP010238:c902586-902251 [Escherichia coli]
MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPYTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGTTKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

>T51686 NZ_CP010238:c902586-902251 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTATACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAACAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9355.74 Da        Isoelectric Point: 4.4665

>AT51686 WP_000581937.1 NZ_CP010238:c902834-902586 [Escherichia coli]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp

>AT51686 NZ_CP010238:c902834-902586 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CQY
PDB 1MVF
PDB 2MRN
PDB 2MRU
AlphaFold DB A0A7U9LMB4

References