Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 4350297..4350935 Replicon chromosome
Accession NZ_CP010237
Organism Escherichia coli strain S43

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag RG71_RS22795 Protein ID WP_000813794.1
Coordinates 4350759..4350935 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag RG71_RS22790 Protein ID WP_077898088.1
Coordinates 4350297..4350713 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RG71_RS22770 4345449..4346390 - 942 WP_001251319.1 ABC transporter permease -
RG71_RS22775 4346391..4347404 - 1014 WP_000220393.1 ABC transporter ATP-binding protein -
RG71_RS22780 4347422..4348567 - 1146 WP_000047429.1 ABC transporter substrate-binding protein -
RG71_RS22785 4348809..4350218 - 1410 WP_000760649.1 PLP-dependent aminotransferase family protein -
RG71_RS22790 4350297..4350713 - 417 WP_077898088.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
RG71_RS22795 4350759..4350935 - 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
RG71_RS22800 4351157..4351387 + 231 WP_000494244.1 YncJ family protein -
RG71_RS22805 4351479..4353440 - 1962 WP_024179169.1 23S rRNA 5-hydroxycytidine C2501 synthase -
RG71_RS22810 4353513..4354049 - 537 WP_000429141.1 DNA-binding transcriptional regulator SutR -
RG71_RS22815 4354102..4355331 + 1230 WP_071531711.1 BenE family transporter YdcO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 4355371..4356519 1148


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T51683 WP_000813794.1 NZ_CP010237:c4350935-4350759 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T51683 NZ_CP010237:c4350935-4350759 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15304.67 Da        Isoelectric Point: 4.6212

>AT51683 WP_077898088.1 NZ_CP010237:c4350713-4350297 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEEAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT51683 NZ_CP010237:c4350713-4350297 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGAGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References