Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 4193236..4193762 Replicon chromosome
Accession NZ_CP010237
Organism Escherichia coli strain S43

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag RG71_RS21930 Protein ID WP_000323025.1
Coordinates 4193475..4193762 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag RG71_RS21925 Protein ID WP_000534858.1
Coordinates 4193236..4193475 (+) Length 80 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RG71_RS21880 4188518..4188844 + 327 WP_000598292.1 YnfA family protein -
RG71_RS21890 4189050..4190273 + 1224 WP_073528452.1 starvation-sensing protein RspA -
RG71_RS21895 4190318..4190566 + 249 Protein_3902 transposase -
RG71_RS21900 4190631..4191782 + 1152 WP_001254932.1 IS30-like element IS30 family transposase -
RG71_RS21910 4191792..4192688 + 897 Protein_3904 IS3 family transposase -
RG71_RS21920 4192906..4193211 - 306 WP_073528454.1 hypothetical protein -
RG71_RS21925 4193236..4193475 + 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
RG71_RS21930 4193475..4193762 + 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
RG71_RS21935 4193834..4193989 + 156 WP_000813254.1 type I toxin-antitoxin system toxin HokD -
RG71_RS21940 4194206..4194457 + 252 WP_000980994.1 hypothetical protein -
RG71_RS21945 4194524..4194802 + 279 WP_012304870.1 hypothetical protein -
RG71_RS21950 4194804..4195853 + 1050 WP_001265198.1 DUF968 domain-containing protein -
RG71_RS21955 4195867..4196619 + 753 WP_001047135.1 antitermination protein -
RG71_RS27430 4196897..4196986 - 90 WP_122985911.1 hypothetical protein -
RG71_RS21960 4197041..4197253 - 213 WP_024172454.1 cold shock-like protein CspF -
RG71_RS21965 4197552..4197767 + 216 WP_000066485.1 cold shock-like protein CspB -
RG71_RS27800 4198131..4198301 - 171 WP_001405948.1 putative zinc-binding protein YnfU -
RG71_RS21970 4198520..4198734 + 215 Protein_3917 phage lysis protein EssD -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 4179727..4209466 29739


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.



Sequences


Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T51681 WP_000323025.1 NZ_CP010237:4193475-4193762 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T51681 NZ_CP010237:4193475-4193762 [Escherichia coli]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT51681 WP_000534858.1 NZ_CP010237:4193236-4193475 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT51681 NZ_CP010237:4193236-4193475 [Escherichia coli]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9


Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References