Detailed information of TA system TA051662

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yafW-ykfI/CbtA-CbeA
• Location: 783256..783935
• Replicon: chromosome
• Accession: NZ_CP010237
• Organism: Escherichia coli strain S43

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: S1FHS4
• Locus tag: RG71_RS04300
• Protein ID: WP_000854680.1
• Coordinates: 783594..783935 (+)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yafW
• Uniprot ID: S1EWR7
• Locus tag: RG71_RS04295
• Protein ID: WP_000070396.1
• Coordinates: 783256..783573 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RG71_RS04250	778694..779515	+	822	WP_001548153.1	DUF945 domain-containing protein	-
RG71_RS04255	779732..780433	+	702	WP_021553244.1	WYL domain-containing protein	-
RG71_RS04260	780474..780710	+	237	WP_001144029.1	hypothetical protein	-
RG71_RS04265	780710..781153	+	444	WP_021553245.1	hypothetical protein	-
RG71_RS04270	781176..781643	+	468	WP_001385283.1	hypothetical protein	-
RG71_RS26580	781720..781959	+	240	Protein_761	DUF905 domain-containing protein	-
RG71_RS04280	782057..782515	+	459	WP_000211838.1	antirestriction protein	-
RG71_RS04285	782531..783007	+	477	WP_000811693.1	RadC family protein	-
RG71_RS04290	783016..783237	+	222	WP_000691994.1	DUF987 domain-containing protein	-
RG71_RS04295	783256..783573	+	318	WP_000070396.1	type IV toxin-antitoxin system antitoxin YafW	Antitoxin
RG71_RS04300	783594..783935	+	342	WP_000854680.1	type IV toxin-antitoxin system toxin YkfI	Toxin
RG71_RS04310	784507..785760	-	1254	WP_000893278.1	glutamate-5-semialdehyde dehydrogenase	-
RG71_RS04315	785772..786875	-	1104	WP_001285288.1	glutamate 5-kinase	-
RG71_RS04320	787163..788218	+	1056	WP_000749863.1	phosphoporin PhoE	-
RG71_RS04325	788257..788658	-	402	WP_000174677.1	sigma factor-binding protein Crl	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	gmhA/lpcA / rhs/PAAR / tssM / tssA / hcp1/tssD1	760064..878595	118531

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (6-104)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12892.95 Da
• Isoelectric Point: 9.6543

>T51662 WP_000854680.1 NZ_CP010237:783594-783935 [Escherichia coli]
MKTLPATTQRAVKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAADILRARQATGLLRQSRNNVVR

Nucleotide

• Length: 342 bp

>T51662 NZ_CP010237:783594-783935 [Escherichia coli]
ATGAAAACTTTACCTGCAACAACTCAGCGGGCGGTGAAGCCCTGCCTGTCACCCGTGGCTGTCTGGCAAATGTTACTGAC
ACGTCTGCTGGAACAGCACTATGGTCTGACAATAAACGACACGCCATTCTGCAATGAGGCTGTGATTAAGGAACACATCG
ATGCCGGTATCACCCTAGCCGATGCCGTGAATTTTCTGGTAGAAAAATACGAGCTGGTTCGTATCGACAGGAAGGGATTT
AGCTGGCAGGAACAATCTCCTTATCTCCGGGCTGCAGACATTCTGCGAGCGCGGCAGGCAACTGGCTTGTTGCGGCAAAG
CCGTAACAACGTAGTACGATGA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11982.62 Da
• Isoelectric Point: 7.0270

>AT51662 WP_000070396.1 NZ_CP010237:783256-783573 [Escherichia coli]
MSNPTRGLQREITLRLGARLVQEGNRLHYLADRASITGKFSDIECRKLDETFPHFIRQMESMLTTGELSPHHAHCVTLYH
NDLTCEADTLGSCGYVYIAIYPTQR

Nucleotide

• Length: 318 bp

>AT51662 NZ_CP010237:783256-783573 [Escherichia coli]
ATGAGCAACCCTACCAGGGGCCTGCAGCGGGAGATTACACTGCGCCTGGGAGCCCGTCTGGTGCAGGAAGGCAACCGACT
GCATTATCTGGCTGACCGGGCCAGCATCACCGGCAAGTTCAGTGACATCGAATGCCGGAAGCTGGATGAAACATTCCCGC
ACTTTATCCGCCAGATGGAATCGATGCTGACCACCGGTGAACTCAGCCCCCACCATGCCCACTGCGTTACCCTGTACCAC
AACGATTTAACCTGCGAAGCCGACACCCTTGGCAGTTGCGGCTACGTATACATCGCCATTTACCCCACTCAGCGTTAA

Structures

Toxin

• AlphaFold DB: S1FHS4

Antitoxin

• AlphaFold DB: S1EWR7