51659

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) sanaTA/AbiEii-DUF6088
Location 4424040..4425333 Replicon chromosome
Accession NZ_CP010236
Organism Escherichia coli strain S42

Toxin (Protein)


Gene name sanaT Uniprot ID A0A3Z8A2R8
Locus tag RG70_RS22995 Protein ID WP_000342496.1
Coordinates 4424428..4425333 (+) Length 302 a.a.

Antitoxin (Protein)


Gene name sanaA Uniprot ID E0J2C6
Locus tag RG70_RS22990 Protein ID WP_000152741.1
Coordinates 4424040..4424435 (+) Length 132 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RG70_RS22975 4420073..4421278 - 1206 WP_001292892.1 restriction endonuclease subunit S -
RG70_RS22980 4421275..4422831 - 1557 WP_024232412.1 type I restriction-modification system subunit M -
RG70_RS22985 4423137..4423970 + 834 WP_001192742.1 DUF4942 domain-containing protein -
RG70_RS22990 4424040..4424435 + 396 WP_000152741.1 type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein Antitoxin
RG70_RS22995 4424428..4425333 + 906 WP_000342496.1 nucleotidyl transferase AbiEii/AbiGii toxin family protein Toxin
RG70_RS23000 4425413..4425598 - 186 WP_073520550.1 hypothetical protein -
RG70_RS23010 4425977..4426627 + 651 WP_001037970.1 HNH endonuclease -
RG70_RS23015 4426635..4427615 - 981 WP_001366032.1 9-O-acetyl-N-acetylneuraminic acid deacetylase -
RG70_RS23020 4427680..4428786 - 1107 WP_001044128.1 N-acetylneuraminate epimerase -
RG70_RS23025 4428806..4429522 - 717 WP_001295734.1 N-acetylneuraminic acid outer membrane channel NanC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - fimB / fimE 4424040..4432654 8614


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Nucleotidyl transferase AbiEii toxin, Type IV TA system

(38-300)

Antitoxin

Protein of unknown function DUF6088

(7-108)


Sequences

Toxin        


Download         Length: 302 a.a.        Molecular weight: 34012.91 Da        Isoelectric Point: 4.7207

>T51659 WP_000342496.1 NZ_CP010236:4424428-4425333 [Escherichia coli]
MDDLTTLFSEVADALGIESVAIVEKDHYIVELLQLLKPLVFDTHQLVFAGGTALAKSGVALNRMSEDVDIKLVPKDAFQA
LSRTQRKNTRKEIVQAVSSAIASSGVFNFDDDYPKVTRDEYRYNDIPVRYPQRVVQAPCLRPFIRLELMEAKLLEPSELR
DIHSLVTGITGKAAIVSAFPCATIISTQAEKLVAMLRRTAAVVRDVERFDDASLVRHIYDTNCIVETSHVDMSLLVDFVQ
QTIEQDIQRYGNQYPQFCASPVDELKMGLDELANNPLYEMRYQQFVSPMVYGERKITWDEA

Download         Length: 906 bp

>T51659 NZ_CP010236:4424428-4425333 [Escherichia coli]
ATGGATGATTTAACCACTCTTTTTTCTGAAGTGGCTGACGCGCTTGGTATTGAGTCTGTCGCAATTGTGGAAAAAGATCA
CTATATCGTTGAGCTTTTGCAATTGCTTAAACCTCTGGTGTTCGATACTCATCAATTGGTGTTTGCCGGAGGAACCGCTC
TGGCAAAATCAGGTGTCGCACTTAATCGCATGTCGGAAGATGTGGATATCAAACTGGTGCCTAAGGATGCATTTCAGGCA
CTTTCCCGCACTCAGCGAAAAAATACCCGTAAAGAAATTGTTCAGGCAGTTTCCAGTGCAATAGCTTCATCGGGTGTGTT
CAATTTTGATGATGATTACCCAAAAGTGACGCGGGATGAATATCGTTATAATGACATACCTGTTCGTTATCCACAGCGAG
TAGTGCAGGCACCTTGTTTGCGTCCTTTTATCCGGCTGGAATTGATGGAAGCGAAGTTACTGGAGCCTTCTGAATTACGG
GATATTCATTCACTGGTAACAGGGATCACGGGAAAAGCGGCTATTGTTTCAGCGTTTCCATGCGCAACTATCATTAGCAC
ACAGGCAGAAAAGCTGGTGGCGATGTTACGTCGTACTGCGGCAGTTGTGCGAGATGTTGAGCGATTTGATGATGCATCGC
TGGTACGTCATATCTACGATACAAACTGTATTGTTGAGACGAGTCATGTTGATATGAGTCTGCTCGTTGATTTTGTTCAA
CAAACTATTGAGCAGGACATTCAGCGTTACGGCAACCAGTACCCGCAGTTTTGCGCGTCTCCAGTTGATGAGCTAAAAAT
GGGACTGGATGAGTTGGCGAACAATCCTCTCTACGAGATGCGCTATCAGCAGTTTGTTTCGCCAATGGTGTATGGAGAGC
GAAAGATCACATGGGATGAGGCTTAA

Antitoxin


Download         Length: 132 a.a.        Molecular weight: 14619.93 Da        Isoelectric Point: 11.6915

>AT51659 WP_000152741.1 NZ_CP010236:4424040-4424435 [Escherichia coli]
MTIKARIQSRLKRSKRYVFTRDDFKDIAGYDQVGRVLRELVREGQLLKVGYGVYTKARRNGITGKVMPAAPGGSSAVIVE
TLERLKVRYRFAGATQAYNSGTSTQIPASPEIKTPRRFKRVLSVGNSRLNG

Download         Length: 396 bp

>AT51659 NZ_CP010236:4424040-4424435 [Escherichia coli]
ATGACGATAAAAGCACGCATTCAGTCACGACTGAAACGTTCTAAACGTTACGTATTCACTCGTGACGACTTTAAAGATAT
TGCAGGCTACGATCAGGTTGGTCGGGTATTGCGCGAGCTTGTCAGGGAAGGGCAACTGTTGAAGGTTGGTTATGGCGTGT
ATACCAAAGCACGGCGGAACGGTATTACAGGCAAGGTAATGCCAGCGGCTCCCGGAGGCTCTTCAGCGGTGATTGTCGAA
ACGCTGGAACGCCTGAAAGTACGCTATCGTTTTGCTGGCGCAACGCAGGCATACAATAGCGGTACTTCTACGCAGATCCC
AGCTTCACCCGAGATAAAAACCCCCCGGCGTTTTAAACGCGTATTATCTGTAGGGAACAGCAGATTAAATGGATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3Z8A2R8

Antitoxin

Source ID Structure
AlphaFold DB A0A1L4ZXD4

References