51652

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 2453474..2454099 Replicon chromosome
Accession NZ_CP010236
Organism Escherichia coli strain S42

Toxin (Protein)


Gene name vapC Uniprot ID U9YZ02
Locus tag RG70_RS12835 Protein ID WP_000911329.1
Coordinates 2453474..2453872 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID U9XQV3
Locus tag RG70_RS12840 Protein ID WP_000450524.1
Coordinates 2453872..2454099 (-) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RG70_RS12815 2449352..2449552 + 201 WP_000383836.1 YpfN family protein -
RG70_RS12820 2449662..2450360 - 699 WP_000679823.1 esterase -
RG70_RS12825 2450434..2452449 - 2016 WP_000829294.1 tRNA cytosine(34) acetyltransferase TmcA -
RG70_RS12830 2452464..2453327 - 864 WP_001267498.1 neutral zinc metallopeptidase -
RG70_RS12835 2453474..2453872 - 399 WP_000911329.1 tRNA(fMet)-specific endonuclease VapC Toxin
RG70_RS12840 2453872..2454099 - 228 WP_000450524.1 toxin-antitoxin system antitoxin VapB Antitoxin
RG70_RS12845 2454254..2454967 - 714 WP_001295467.1 phosphoribosylaminoimidazolesuccinocarboxamide synthase -
RG70_RS12855 2455180..2456214 - 1035 WP_001297320.1 outer membrane protein assembly factor BamC -
RG70_RS12860 2456231..2457109 - 879 WP_001295469.1 4-hydroxy-tetrahydrodipicolinate synthase -
RG70_RS12865 2457255..2457827 + 573 WP_000176191.1 glycine cleavage system transcriptional repressor -
RG70_RS12870 2457827..2458297 + 471 WP_001068682.1 thioredoxin-dependent thiol peroxidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-125)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14924.23 Da        Isoelectric Point: 8.5325

>T51652 WP_000911329.1 NZ_CP010236:c2453872-2453474 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLEVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Download         Length: 399 bp

>T51652 NZ_CP010236:c2453872-2453474 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTGATTGAAGGTTTCGTATCTCGTTTGGAAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGATTGGAGCTAA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8498.61 Da        Isoelectric Point: 4.3867

>AT51652 WP_000450524.1 NZ_CP010236:c2454099-2453872 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Download         Length: 228 bp

>AT51652 NZ_CP010236:c2454099-2453872 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAGGATGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XW84

Antitoxin

Source ID Structure
AlphaFold DB A0A829CM33

References