Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 1023364..1024018 Replicon chromosome
Accession NZ_CP010235
Organism Escherichia coli strain S40

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag RG69_RS05370 Protein ID WP_000244777.1
Coordinates 1023611..1024018 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag RG69_RS05365 Protein ID WP_000354046.1
Coordinates 1023364..1023630 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RG69_RS05340 1018533..1019276 + 744 WP_000951964.1 SDR family oxidoreductase -
RG69_RS05345 1019333..1020766 - 1434 WP_001307385.1 6-phospho-beta-glucosidase BglA -
RG69_RS05350 1020811..1021122 + 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
RG69_RS05355 1021286..1021945 + 660 WP_000250274.1 hemolysin III family protein -
RG69_RS05360 1022141..1023121 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
RG69_RS05365 1023364..1023630 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
RG69_RS05370 1023611..1024018 + 408 WP_000244777.1 toxin CptA Toxin
RG69_RS05375 1024058..1024579 - 522 WP_001055874.1 flavodoxin FldB -
RG69_RS05380 1024691..1025587 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
RG69_RS05385 1025612..1026322 + 711 WP_000715208.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
RG69_RS05390 1026328..1028061 + 1734 WP_000813223.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T51616 WP_000244777.1 NZ_CP010235:1023611-1024018 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T51616 NZ_CP010235:1023611-1024018 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT51616 WP_000354046.1 NZ_CP010235:1023364-1023630 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT51616 NZ_CP010235:1023364-1023630 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References