Detailed information of TA system TA051612

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-Phd
• Location: 10900..11501
• Replicon: plasmid C
• Accession: NZ_CP010234
• Organism: Escherichia coli strain S30

Toxin (Protein)

• Gene name: doc
• Uniprot ID: -
• Locus tag: RG68_RS26640
• Protein ID: WP_073546715.1
• Coordinates: 10900..11280 (-)
• Length: 127 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: U9YQH9
• Locus tag: RG68_RS26645
• Protein ID: WP_001190712.1
• Coordinates: 11280..11501 (-)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RG68_RS28115	6340..7038	-	699	Protein_7	IS1 family transposase	-
RG68_RS26595	7209..7418	+	210	WP_073546712.1	c1 repressor inactivator	-
RG68_RS26600	7529..8380	+	852	WP_073546713.1	hypothetical protein	-
RG68_RS26605	8413..8991	-	579	Protein_10	phage antirepressor KilAC domain-containing protein	-
RG68_RS28120	9016..9714	-	699	Protein_11	IS1 family transposase	-
RG68_RS26630	9771..10688	-	918	Protein_12	DUF968 domain-containing protein	-
RG68_RS26635	10716..10895	-	180	WP_038997082.1	hypothetical protein	-
RG68_RS26640	10900..11280	-	381	WP_073546715.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
RG68_RS26645	11280..11501	-	222	WP_001190712.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
RG68_RS26650	11660..12727	+	1068	Protein_16	type I restriction-modification system subunit M	-
RG68_RS26655	12724..13227	+	504	Protein_17	restriction endonuclease subunit S	-
RG68_RS28130	13283..13981	+	699	Protein_18	IS1 family transposase	-
RG68_RS26675	13996..14790	+	795	Protein_19	restriction endonuclease subunit S	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	-	1..117266	117266
	-	inside	IScluster/Tn	-	-	818..13961	13143

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Fido domain (4-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-51)

Sequences

Toxin

Protein

• Length: 127 a.a.
• Molecular weight: 13663.41 Da
• Isoelectric Point: 5.6342

>T51612 WP_073546715.1 NZ_CP010234:c11280-10900 [Escherichia coli]
MRHISPEELIALHDANINRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELAYLTVGAATGEISVSSVADTLRRLYGSAE

Nucleotide

• Length: 381 bp

>T51612 NZ_CP010234:c11280-10900 [Escherichia coli]
ATGAGGCATATATCACCGGAAGAACTTATTGCGCTTCATGATGCGAATATAAACCGCTACGGCGGCCTGCCGGGCATGTC
AGATCCGGGTAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCCACCTACCTGGTGGCTACAGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
CTGTTATTTCTACGCCGTAACGGGGTGCAGGTATTTGATTCACCTGAACTGGCATACCTTACCGTAGGGGCTGCGACCGG
AGAGATATCTGTATCTTCTGTCGCCGACACGTTACGTAGATTGTATGGTTCTGCGGAGTAG

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8133.10 Da
• Isoelectric Point: 4.7708

>AT51612 WP_001190712.1 NZ_CP010234:c11501-11280 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Nucleotide

• Length: 222 bp

>AT51612 NZ_CP010234:c11501-11280 [Escherichia coli]
ATGCAATCCATTAACTTCCGTACCGCGCGTGGCAACCTTTCTGAAGTGCTCAACAATGTTGAAGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACACTGGACTCCACCAACAAGGAACTGGTTAACCGATAA

Structures

Antitoxin

• AlphaFold DB: A0A829CJB6