Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) parDE/RelE-RHH
Location 54067..54614 Replicon plasmid B
Accession NZ_CP010233
Organism Escherichia coli strain S30

Toxin (Protein)


Gene name parE Uniprot ID W0LWB3
Locus tag RG68_RS26005 Protein ID WP_000970007.1
Coordinates 54067..54348 (-) Length 94 a.a.

Antitoxin (Protein)


Gene name parD Uniprot ID E1QMW3
Locus tag RG68_RS26010 Protein ID WP_000079941.1
Coordinates 54345..54614 (-) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RG68_RS25975 50694..51296 + 603 WP_000517695.1 hypothetical protein -
RG68_RS25980 51352..51846 + 495 WP_001459505.1 hypothetical protein -
RG68_RS29230 51846..52112 + 267 WP_000955263.1 hypothetical protein -
RG68_RS25995 52589..53062 - 474 WP_001334658.1 hypothetical protein -
RG68_RS26000 53179..54027 - 849 WP_001057996.1 3'-5' exonuclease -
RG68_RS26005 54067..54348 - 282 WP_000970007.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
RG68_RS26010 54345..54614 - 270 WP_000079941.1 hypothetical protein Antitoxin
RG68_RS29070 54669..54827 - 159 WP_000841008.1 hypothetical protein -
RG68_RS26025 55523..56554 - 1032 WP_000907873.1 replication initiation protein -
RG68_RS26040 57527..57790 + 264 WP_001324596.1 ash family protein -
RG68_RS26045 57708..57995 + 288 WP_000074849.1 conjugal transfer protein TraA -
RG68_RS26055 58437..58970 + 534 WP_001303319.1 transcription termination factor NusG -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid tet(A) / aph(6)-Id / aph(3'')-Ib / sul2 / fosA3 / aph(3')-Ia - 1..136060 136060


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-92)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 94 a.a.        Molecular weight: 10892.63 Da        Isoelectric Point: 9.7617

>T51607 WP_000970007.1 NZ_CP010233:c54348-54067 [Escherichia coli]
MMEIFWTMLASQDRKRIRKYIAEQNLMAAIELDERIGYSASSLAGQPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKV
YILRVLHTAQKWP

Download         Length: 282 bp

>T51607 NZ_CP010233:c54348-54067 [Escherichia coli]
ATGATGGAGATATTCTGGACCATGCTGGCCAGCCAGGACAGGAAGCGCATTCGTAAGTACATTGCGGAGCAGAACCTGAT
GGCCGCCATTGAACTGGACGAACGGATTGGTTATTCAGCCAGCAGCCTGGCCGGGCAGCCGTATAAAGGCCGCAACGGTC
GTGTGGAAGGTACCCGGGAACTGGTTATTCACCCGCATTTTGTTCTGGTTTATGAGGTCGACAGCCAGTGGGGAAAAGTG
TATATCCTGCGCGTGTTGCATACTGCACAGAAGTGGCCATAG

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10252.58 Da        Isoelectric Point: 7.2057

>AT51607 WP_000079941.1 NZ_CP010233:c54614-54345 [Escherichia coli]
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQHNTWFRDQVEAGRQQLERGDVLPHDMVESSAAAWRD
EMSRKVAGK

Download         Length: 270 bp

>AT51607 NZ_CP010233:c54614-54345 [Escherichia coli]
ATGGCACAGGTTAATATGAGTTTAAGAATCGACGCTGAACTGAAGGATGCTTTTATGGCCGCTGCAAAAAGCATGGACCG
TAATGGCTCTCAGTTAATTCGGGATTTTATGCGCCAGACCGTTGAACGGCAGCATAATACCTGGTTCCGTGACCAGGTTG
AGGCAGGACGTCAGCAACTCGAGCGCGGCGATGTGCTTCCCCATGACATGGTCGAATCTTCCGCTGCTGCATGGCGGGAT
GAAATGAGCAGGAAGGTTGCCGGTAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7G1GDN5

Antitoxin

Source ID Structure
AlphaFold DB A0A629TXC5

References