Detailed information of TA system TA051598

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 3826000..3826835
• Replicon: chromosome
• Accession: NZ_CP010231
• Organism: Escherichia coli strain S30

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: B1LDL9
• Locus tag: RG68_RS20070
• Protein ID: WP_001094444.1
• Coordinates: 3826458..3826835 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: B7LDN9
• Locus tag: RG68_RS20065
• Protein ID: WP_001285607.1
• Coordinates: 3826000..3826368 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RG68_RS20030	3821892..3822572	+	681	WP_001282919.1	WYL domain-containing protein	-
RG68_RS20035	3822720..3823397	+	678	WP_001097301.1	hypothetical protein	-
RG68_RS20040	3823427..3823636	+	210	WP_032150870.1	DUF905 family protein	-
RG68_RS20045	3823726..3824544	+	819	WP_001175175.1	DUF945 domain-containing protein	-
RG68_RS20050	3824636..3825121	+	486	WP_000213722.1	antirestriction protein	-
RG68_RS20055	3825136..3825612	+	477	WP_001384029.1	RadC family protein	-
RG68_RS20060	3825699..3825920	+	222	WP_000692350.1	DUF987 domain-containing protein	-
RG68_RS20065	3826000..3826368	+	369	WP_001285607.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
RG68_RS20070	3826458..3826835	+	378	WP_001094444.1	TA system toxin CbtA family protein	Toxin
RG68_RS20075	3826832..3827185	+	354	Protein_3579	hypothetical protein	-
RG68_RS20090	3828601..3828798	+	198	WP_001327226.1	DUF957 domain-containing protein	-
RG68_RS29175	3828883..3829029	+	147	WP_001290178.1	hypothetical protein	-
RG68_RS20105	3830074..3830724	+	651	WP_001037966.1	HNH endonuclease	-
RG68_RS20110	3830732..3831712	-	981	WP_001295601.1	9-O-acetyl-N-acetylneuraminic acid deacetylase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	fimB / fimE	3795077..3838084	43007

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (19-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14087.02 Da
• Isoelectric Point: 7.3523

>T51598 WP_001094444.1 NZ_CP010231:3826458-3826835 [Escherichia coli]
MNTLPDTHVREASHCQSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SVCTRSQLINSIDILRARRATGLMARDNYRTVNNITLGKHPGAKQ

Nucleotide

• Length: 378 bp

>T51598 NZ_CP010231:3826458-3826835 [Escherichia coli]
ATGAACACATTACCCGATACACACGTACGGGAGGCATCGCATTGCCAGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCATTACGGCCTCACACTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTTGAAAAATACGCACTGGTGCGTACCGACCAGCCGGGATTC
AGCGTCTGTACTCGTTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCCCGCCGGGCAACCGGCCTGATGGCCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAACATCCGGGGGCGAAACAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13689.53 Da
• Isoelectric Point: 6.4669

>AT51598 WP_001285607.1 NZ_CP010231:3826000-3826368 [Escherichia coli]
VSDTLPGTTLPDDNNDRTWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKRLTCEADTLGSCGYVYMAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT51598 NZ_CP010231:3826000-3826368 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACGCTTCCCGACGACAATAACGACCGCACCTGGTGGGGACTGCCCTGCACCGTGAC
GCCCTGTTTTGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCAGACCGCGCCGGTATCAGAGGCCGGT
TCAGCGACGCGGATGCGTACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAAGGCTGACCTGCGAAGCCGACACCCTCGGCAGTTG
TGGCTACGTTTATATGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: B1LDL9

Antitoxin

• AlphaFold DB: A0A1M0JLU8