Detailed information of TA system TA051597

Bioinformatically predicted

Overview

TA module

• Type: V
• Classification (family/domain): ghoTS/ghoT-GhoS
• Location: 3642411..3642908
• Replicon: chromosome
• Accession: NZ_CP010231
• Organism: Escherichia coli strain S30

Toxin (Protein)

• Gene name: ghoT
• Uniprot ID: L4JJG6
• Locus tag: RG68_RS19025
• Protein ID: WP_001173343.1
• Coordinates: 3642735..3642908 (+)
• Length: 58 a.a.

Antitoxin (Protein)

• Gene name: ghoS
• Uniprot ID: -
• Locus tag: RG68_RS19020
• Protein ID: WP_073546616.1
• Coordinates: 3642411..3642707 (+)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RG68_RS18995	3639142..3639861	-	720	WP_000611288.1	two-component system response regulator DcuR	-
RG68_RS19000	3639858..3641489	-	1632	WP_001216477.1	sensor histidine kinase	-
RG68_RS19005	3641670..3641900	+	231	WP_000371704.1	(4Fe-4S)-binding protein	-
RG68_RS19010	3641912..3642184	+	273	WP_000405651.1	N-acetyltransferase	-
RG68_RS19020	3642411..3642707	+	297	WP_073546616.1	type V toxin-antitoxin system endoribonuclease antitoxin GhoS	Antitoxin
RG68_RS19025	3642735..3642908	+	174	WP_001173343.1	type V toxin-antitoxin system toxin GhoT	Toxin
RG68_RS19030	3643027..3644544	-	1518	WP_001295074.1	lysine--tRNA ligase	-
RG68_RS19035	3644781..3646238	-	1458	WP_000856829.1	dipeptide/tripeptide permease DtpC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin GhoT/OrtT (4-57)

Antitoxin

• Endoribonuclease antitoxin GhoS (5-94)

Sequences

Toxin

Protein

• Length: 58 a.a.
• Molecular weight: 6555.06 Da
• Isoelectric Point: 10.2288

>T51597 WP_001173343.1 NZ_CP010231:3642735-3642908 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Nucleotide

• Length: 174 bp

>T51597 NZ_CP010231:3642735-3642908 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 11466.99 Da
• Isoelectric Point: 4.2544

>AT51597 WP_073546616.1 NZ_CP010231:3642411-3642707 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFNLNIMTVDDYFRQFYK

Nucleotide

• Length: 297 bp

>AT51597 NZ_CP010231:3642411-3642707 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGATT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTAACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Structures

Toxin

• AlphaFold DB: A0A7U9QI41