Detailed information of TA system TA051590

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 1381871..1382703
• Replicon: chromosome
• Accession: NZ_CP010231
• Organism: Escherichia coli strain S30

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A7ZVJ9
• Locus tag: RG68_RS07380
• Protein ID: WP_000854765.1
• Coordinates: 1382329..1382703 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: S1NM52
• Locus tag: RG68_RS07375
• Protein ID: WP_001295723.1
• Coordinates: 1381871..1382239 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RG68_RS07335	1378986..1379144	+	159	WP_001323397.1	DUF905 family protein	-
RG68_RS07345	1379299..1380117	+	819	WP_001234652.1	DUF945 domain-containing protein	-
RG68_RS07355	1380459..1380932	+	474	WP_073546515.1	antirestriction protein	-
RG68_RS07360	1380948..1381424	+	477	WP_001186774.1	RadC family protein	-
RG68_RS07365	1381487..1381708	+	222	WP_000692345.1	DUF987 domain-containing protein	-
RG68_RS07375	1381871..1382239	+	369	WP_001295723.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
RG68_RS07380	1382329..1382703	+	375	WP_000854765.1	TA system toxin CbtA family protein	Toxin
RG68_RS07385	1382700..1382906	+	207	WP_000976829.1	hypothetical protein	-
RG68_RS28335	1382919..1383032	+	114	WP_001161660.1	DUF957 domain-containing protein	-
RG68_RS07395	1383296..1384042	-	747	Protein_1336	IS21-like element ISEc12 family helper ATPase IstB	-
RG68_RS07400	1384057..1385598	-	1542	WP_001445980.1	IS21-like element ISEc12 family transposase	-
RG68_RS29130	1386416..1386805	+	390	WP_077249349.1	transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13970.98 Da
• Isoelectric Point: 7.2909

>T51590 WP_000854765.1 NZ_CP010231:1382329-1382703 [Escherichia coli]
MKTLPDTHVREVSCCPSPVTIWQTLLTRLLDQHYSLTLNDTPFVDERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SAGASSQLINSIDILRARRATGLMTRHNYRTVNNITLGKYPEAK

Nucleotide

• Length: 375 bp

>T51590 NZ_CP010231:1382329-1382703 [Escherichia coli]
ATGAAAACATTACCCGACACGCATGTACGGGAGGTATCGTGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGGTTACTGGACCAGCATTACAGCCTTACGCTGAATGACACACCGTTCGTGGATGAACGCGTGATTGAGCAGCATATTG
AGGCAGGTATTTCACTGTGTGATGCAGTGAACTTTCTCGTGGAAAAATACGCACTGGTACGAACTGACCAGCCGGGATTC
AGCGCAGGAGCCTCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCCGGGCAACAGGCCTGATGACCCGCCA
CAACTACAGAACGGTGAACAATATCACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13592.40 Da
• Isoelectric Point: 6.6255

>AT51590 WP_001295723.1 NZ_CP010231:1381871-1382239 [Escherichia coli]
VSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFRDADAYPLDQAFPLLMKQLKLMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT51590 NZ_CP010231:1381871-1382239 [Escherichia coli]
GTGTCAGACACACTTTCCGGGACAACGCATCCCGATGACAATGACGACCATCCCTGGTGGGGACTACCCTGCACAGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCGGT
TCAGAGATGCGGATGCGTACCCTCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGAAATTAATGCTCACCAGTGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGACCTGTGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCTGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A7ZVJ9

Antitoxin

• AlphaFold DB: S1NM52