Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 776639..777277 Replicon chromosome
Accession NZ_CP010231
Organism Escherichia coli strain S30

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag RG68_RS04060 Protein ID WP_000813794.1
Coordinates 776639..776815 (+) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag RG68_RS04065 Protein ID WP_001270286.1
Coordinates 776861..777277 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RG68_RS04040 772258..773472 - 1215 WP_071597387.1 BenE family transporter YdcO -
RG68_RS04045 773525..774061 + 537 WP_073546468.1 DNA-binding transcriptional regulator SutR -
RG68_RS04050 774134..776095 + 1962 WP_001301045.1 23S rRNA 5-hydroxycytidine C2501 synthase -
RG68_RS04055 776187..776417 - 231 WP_000494244.1 YncJ family protein -
RG68_RS04060 776639..776815 + 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
RG68_RS04065 776861..777277 + 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
RG68_RS04070 777356..778762 + 1407 WP_000760585.1 PLP-dependent aminotransferase family protein -
RG68_RS04075 779007..780152 + 1146 WP_000047424.1 ABC transporter substrate-binding protein -
RG68_RS04080 780170..781183 + 1014 WP_000220399.1 ABC transporter ATP-binding protein -
RG68_RS04085 781184..782125 + 942 WP_001251313.1 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 770953..772218 1265


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T51581 WP_000813794.1 NZ_CP010231:776639-776815 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T51581 NZ_CP010231:776639-776815 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT51581 WP_001270286.1 NZ_CP010231:776861-777277 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT51581 NZ_CP010231:776861-777277 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References