Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4605416..4606034 Replicon chromosome
Accession NZ_CP010230
Organism Escherichia coli strain S21

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag RG67_RS23640 Protein ID WP_001291435.1
Coordinates 4605416..4605634 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag RG67_RS23645 Protein ID WP_000344800.1
Coordinates 4605660..4606034 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RG67_RS23605 4600705..4601277 + 573 WP_000779826.1 lipoprotein -
RG67_RS23610 4601308..4601619 - 312 WP_000409911.1 MGMT family protein -
RG67_RS23620 4601998..4602351 + 354 WP_000878141.1 DUF1428 family protein -
RG67_RS23625 4602393..4603943 - 1551 WP_001299455.1 cyclic-guanylate-specific phosphodiesterase PdeB -
RG67_RS23630 4604107..4604577 - 471 WP_000136192.1 YlaC family protein -
RG67_RS23635 4604693..4605244 - 552 WP_000102564.1 maltose O-acetyltransferase -
RG67_RS23640 4605416..4605634 - 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
RG67_RS23645 4605660..4606034 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
RG67_RS23650 4606580..4609729 - 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -
RG67_RS23655 4609752..4610945 - 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T51580 WP_001291435.1 NZ_CP010230:c4605634-4605416 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T51580 NZ_CP010230:c4605634-4605416 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT51580 WP_000344800.1 NZ_CP010230:c4606034-4605660 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT51580 NZ_CP010230:c4606034-4605660 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References