Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) chpSB/PRK09812-ChpS
Location 3947284..3947879 Replicon chromosome
Accession NZ_CP010230
Organism Escherichia coli strain S21

Toxin (Protein)


Gene name chpB Uniprot ID A0A3L3YDL1
Locus tag RG67_RS20455 Protein ID WP_000239576.1
Coordinates 3947529..3947879 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name chpS Uniprot ID U9Y2K1
Locus tag RG67_RS20450 Protein ID WP_001223208.1
Coordinates 3947284..3947535 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RG67_RS20440 3942949..3946728 + 3780 WP_000060896.1 autotransporter assembly complex protein TamB -
RG67_RS20445 3946731..3947072 + 342 WP_001219160.1 gamma-glutamylcyclotransferase -
RG67_RS20450 3947284..3947535 + 252 WP_001223208.1 type II toxin-antitoxin system antitoxin ChpS Antitoxin
RG67_RS20455 3947529..3947879 + 351 WP_000239576.1 endoribonuclease toxin ChpB Toxin
RG67_RS20460 3947959..3948489 - 531 WP_000055075.1 inorganic diphosphatase -
RG67_RS20465 3948799..3949755 + 957 WP_000265933.1 galactofuranose ABC transporter substrate-binding protein YtfQ -
RG67_RS20470 3949895..3951397 + 1503 WP_000205795.1 sugar ABC transporter ATP-binding protein -
RG67_RS20475 3951411..3952433 + 1023 WP_001296689.1 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(8-114)

Antitoxin

SpoVT-AbrB domain

(6-49)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12566.53 Da        Isoelectric Point: 6.2232

>T51577 WP_000239576.1 NZ_CP010230:3947529-3947879 [Escherichia coli]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPIRQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLRARLAKRIGLAADEVVEEALLRLQAVVE

Download         Length: 351 bp

>T51577 NZ_CP010230:3947529-3947879 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTAGGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9271.58 Da        Isoelectric Point: 4.4201

>AT51577 WP_001223208.1 NZ_CP010230:3947284-3947535 [Escherichia coli]
MRITIKRWGNSAGMVIPNIVMKELNLQPGQSVEAQVSNNQLILTPISRRYSLDELLAQCDMNAAELSEQDVWGKSTPAGD
EIW

Download         Length: 252 bp

>AT51577 NZ_CP010230:3947284-3947535 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAAGCGCAGGTGAGCAACAACCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3L3YDL1

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LQ26

References