Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 2939512..2940130 Replicon chromosome
Accession NZ_CP010228
Organism Escherichia coli strain S3

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag RG65_RS15490 Protein ID WP_001291435.1
Coordinates 2939912..2940130 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag RG65_RS15485 Protein ID WP_000344800.1
Coordinates 2939512..2939886 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RG65_RS15475 2934601..2935794 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
RG65_RS15480 2935817..2938966 + 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -
RG65_RS15485 2939512..2939886 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
RG65_RS15490 2939912..2940130 + 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
RG65_RS15495 2940301..2940852 + 552 WP_000102564.1 maltose O-acetyltransferase -
RG65_RS15500 2940968..2941438 + 471 WP_000136192.1 YlaC family protein -
RG65_RS15505 2941602..2943152 + 1551 WP_001299455.1 cyclic-guanylate-specific phosphodiesterase PdeB -
RG65_RS15510 2943194..2943547 - 354 WP_000878140.1 DUF1428 domain-containing protein -
RG65_RS15520 2943926..2944261 + 336 WP_187658848.1 MGMT family protein -
RG65_RS15525 2944268..2944840 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T51533 WP_001291435.1 NZ_CP010228:2939912-2940130 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T51533 NZ_CP010228:2939912-2940130 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTATACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT51533 WP_000344800.1 NZ_CP010228:2939512-2939886 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT51533 NZ_CP010228:2939512-2939886 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References