Detailed information of TA system TA051532

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 1927220..1927858
• Replicon: chromosome
• Accession: NZ_CP010228
• Organism: Escherichia coli strain S3

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: RG65_RS10135
• Protein ID: WP_000813794.1
• Coordinates: 1927682..1927858 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: RG65_RS10130
• Protein ID: WP_001270286.1
• Coordinates: 1927220..1927636 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RG65_RS10110	1922372..1923313	-	942	WP_073507375.1	ABC transporter permease	-
RG65_RS10115	1923314..1924327	-	1014	WP_000220396.1	ABC transporter ATP-binding protein	-
RG65_RS10120	1924345..1925490	-	1146	WP_073507376.1	ABC transporter substrate-binding protein	-
RG65_RS10125	1925732..1927141	-	1410	WP_073507377.1	PLP-dependent aminotransferase family protein	-
RG65_RS10130	1927220..1927636	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
RG65_RS10135	1927682..1927858	-	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
RG65_RS10140	1928080..1928310	+	231	WP_000494244.1	YncJ family protein	-
RG65_RS10145	1928402..1930363	-	1962	WP_001307191.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
RG65_RS10150	1930436..1930972	-	537	WP_000429141.1	DNA-binding transcriptional regulator SutR	-
RG65_RS10155	1931025..1932239	+	1215	WP_073507378.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	1932279..1933427	1148

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T51532 WP_000813794.1 NZ_CP010228:c1927858-1927682 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T51532 NZ_CP010228:c1927858-1927682 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT51532 WP_001270286.1 NZ_CP010228:c1927636-1927220 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT51532 NZ_CP010228:c1927636-1927220 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• PDB: 6HPB