Detailed information of TA system TA051514

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yafW-ykfI/CbtA-CbeA
• Location: 3173509..3174188
• Replicon: chromosome
• Accession: NZ_CP010226
• Organism: Escherichia coli strain S1

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: -
• Locus tag: RG64_RS17335
• Protein ID: WP_032293845.1
• Coordinates: 3173847..3174188 (+)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yafW
• Uniprot ID: S1EWR7
• Locus tag: RG64_RS17330
• Protein ID: WP_000070396.1
• Coordinates: 3173509..3173826 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RG64_RS17285	3168947..3169768	+	822	WP_000197387.1	DUF945 domain-containing protein	-
RG64_RS17290	3169985..3170686	+	702	WP_000189411.1	WYL domain-containing protein	-
RG64_RS17295	3170727..3170963	+	237	WP_001144031.1	hypothetical protein	-
RG64_RS17300	3170963..3171406	+	444	WP_000649865.1	hypothetical protein	-
RG64_RS17305	3171429..3171896	+	468	WP_001385283.1	hypothetical protein	-
RG64_RS17310	3171973..3172212	+	240	WP_000194654.1	DUF905 domain-containing protein	-
RG64_RS17315	3172310..3172768	+	459	WP_000211838.1	antirestriction protein	-
RG64_RS17320	3172784..3173260	+	477	WP_000811693.1	RadC family protein	-
RG64_RS17325	3173269..3173490	+	222	WP_000691994.1	DUF987 domain-containing protein	-
RG64_RS17330	3173509..3173826	+	318	WP_000070396.1	type IV toxin-antitoxin system antitoxin YafW	Antitoxin
RG64_RS17335	3173847..3174188	+	342	WP_032293845.1	type IV toxin-antitoxin system toxin YkfI	Toxin
RG64_RS17340	3174854..3175063	+	210	Protein_3063	integrase core domain-containing protein	-
RG64_RS17345	3175105..3175848	-	744	WP_032177817.1	AraC family transcriptional regulator	-
RG64_RS17355	3176236..3177147	-	912	WP_073527469.1	IS5 family transposase	-
RG64_RS17360	3177500..3178564	+	1065	WP_072003692.1	acyltransferase	-
RG64_RS17365	3178596..3178961	-	366	WP_077758612.1	tail fiber assembly protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	gmhA/lpcA	3155188..3234821	79633

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (6-104)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12888.96 Da
• Isoelectric Point: 9.6543

>T51514 WP_032293845.1 NZ_CP010226:3173847-3174188 [Escherichia coli]
MKTLPATTQRAVKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGIPLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAADILRARQATGLLRQSRNNVVR

Nucleotide

• Length: 342 bp

>T51514 NZ_CP010226:3173847-3174188 [Escherichia coli]
ATGAAAACTTTACCTGCAACAACTCAGCGGGCGGTGAAGCCCTGCCTGTCACCCGTGGCTGTCTGGCAAATGTTACTGAC
ACGTCTGCTGGAACAGCACTATGGTCTGACAATAAACGACACGCCATTCTGCAATGAGGCAGTGATTAAGGAACACATCG
ATGCCGGTATCCCCCTAGCCGATGCCGTGAATTTTCTGGTAGAAAAATACGAGCTGGTTCGTATCGACAGGAAGGGATTT
AGCTGGCAGGAACAATCTCCTTATCTCCGGGCTGCAGACATTCTGCGAGCGCGGCAGGCAACTGGCTTGTTGCGGCAAAG
CCGTAACAACGTAGTACGATGA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11982.62 Da
• Isoelectric Point: 7.0270

>AT51514 WP_000070396.1 NZ_CP010226:3173509-3173826 [Escherichia coli]
MSNPTRGLQREITLRLGARLVQEGNRLHYLADRASITGKFSDIECRKLDETFPHFIRQMESMLTTGELSPHHAHCVTLYH
NDLTCEADTLGSCGYVYIAIYPTQR

Nucleotide

• Length: 318 bp

>AT51514 NZ_CP010226:3173509-3173826 [Escherichia coli]
ATGAGCAACCCTACCAGGGGCCTGCAGCGGGAGATTACACTGCGCCTGGGAGCCCGTCTGGTGCAGGAAGGCAACCGACT
GCATTATCTGGCTGACCGGGCCAGCATCACCGGCAAGTTCAGTGACATCGAATGCCGGAAGCTGGATGAAACATTCCCGC
ACTTTATCCGCCAGATGGAATCGATGCTGACCACCGGTGAACTCAGCCCCCACCATGCCCACTGCGTTACCCTGTACCAC
AACGATTTAACCTGCGAAGCCGACACCCTTGGCAGTTGCGGCTACGTATACATCGCCATTTACCCCACTCAGCGTTAA

Structures

Antitoxin

• AlphaFold DB: S1EWR7