51513

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 2974950..2975568 Replicon chromosome
Accession NZ_CP010226
Organism Escherichia coli strain S1

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag RG64_RS16175 Protein ID WP_001291435.1
Coordinates 2975350..2975568 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag RG64_RS16170 Protein ID WP_000344800.1
Coordinates 2974950..2975324 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RG64_RS16160 2970039..2971232 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
RG64_RS16165 2971255..2974404 + 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -
RG64_RS16170 2974950..2975324 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
RG64_RS16175 2975350..2975568 + 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
RG64_RS16180 2975740..2976290 + 551 Protein_2863 maltose O-acetyltransferase -
RG64_RS16185 2976406..2976876 + 471 WP_000136192.1 YlaC family protein -
RG64_RS16190 2977040..2978590 + 1551 WP_001310610.1 cyclic-guanylate-specific phosphodiesterase PdeB -
RG64_RS16195 2978632..2978985 - 354 WP_000878140.1 DUF1428 domain-containing protein -
RG64_RS16205 2979364..2979675 + 312 WP_000409911.1 MGMT family protein -
RG64_RS16210 2979706..2980278 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T51513 WP_001291435.1 NZ_CP010226:2975350-2975568 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T51513 NZ_CP010226:2975350-2975568 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTTTGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT51513 WP_000344800.1 NZ_CP010226:2974950-2975324 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT51513 NZ_CP010226:2974950-2975324 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References