Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 1836478..1837281 Replicon chromosome
Accession NZ_CP010226
Organism Escherichia coli strain S1

Toxin (Protein)


Gene name KacT Uniprot ID A0A8B2S6X5
Locus tag RG64_RS10015 Protein ID WP_048213819.1
Coordinates 1836478..1837008 (-) Length 177 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID A0A8B2S700
Locus tag RG64_RS10020 Protein ID WP_048213818.1
Coordinates 1837012..1837281 (-) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RG64_RS09980 1831995..1832276 - 282 WP_000639434.1 hypothetical protein -
RG64_RS09985 1832425..1832670 + 246 WP_160448951.1 hypothetical protein -
RG64_RS09990 1832728..1833516 - 789 WP_001543756.1 site-specific integrase -
RG64_RS27065 1833536..1834207 - 672 WP_000796235.1 hypothetical protein -
RG64_RS10005 1835139..1835384 + 246 WP_048213821.1 hypothetical protein -
RG64_RS10010 1835389..1836393 + 1005 WP_073527372.1 hypothetical protein -
RG64_RS10015 1836478..1837008 - 531 WP_048213819.1 GNAT family N-acetyltransferase Toxin
RG64_RS10020 1837012..1837281 - 270 WP_048213818.1 DUF1778 domain-containing protein Antitoxin
RG64_RS10025 1837496..1837993 - 498 Protein_1785 IS3 family transposase -
RG64_RS10030 1838074..1839582 - 1509 WP_001189109.1 group II intron reverse transcriptase/maturase -
RG64_RS25955 1840255..1840940 - 686 Protein_1787 IS3 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 1831211..1848490 17279
- flank IS/Tn - - 1831211..1831486 275


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(92-156)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 177 a.a.        Molecular weight: 20322.17 Da        Isoelectric Point: 6.7544

>T51509 WP_048213819.1 NZ_CP010226:c1837008-1836478 [Escherichia coli]
MDGLRIEIFSEEVEYELSNFDCGEEYLNTFLTDHLKRQHNSKILRGYLLVTREDKPRVMGYYTLSGSCFEKTLLPSKTQQ
KRVPYKNVPSVTLGRLAIDKSIHHQGYGETLVTHAMKVVYQASQAVGIHGMFVEALNDNAKKFYLRLGFIQLKEENSNSL
FYPTKSVEELFEVKDE

Download         Length: 531 bp

>T51509 NZ_CP010226:c1837008-1836478 [Escherichia coli]
ATGGACGGTTTAAGGATTGAGATTTTTTCAGAGGAAGTTGAATATGAATTAAGCAACTTCGATTGTGGAGAAGAGTATCT
GAATACTTTTCTTACTGACCATCTGAAACGCCAGCACAACAGTAAGATTTTGCGTGGCTATCTGCTCGTGACGAGAGAGG
ATAAACCACGTGTTATGGGGTACTACACGCTGTCTGGCAGCTGCTTTGAGAAAACACTTCTACCCAGCAAAACGCAACAA
AAGCGCGTCCCCTACAAAAATGTGCCGAGCGTGACTTTAGGCCGTCTGGCAATTGATAAAAGCATTCATCACCAGGGATA
TGGAGAAACACTTGTTACCCATGCTATGAAAGTCGTGTATCAGGCCTCGCAGGCTGTGGGTATACACGGAATGTTCGTGG
AAGCGCTGAACGACAACGCCAAAAAATTCTACCTTCGCCTGGGTTTTATACAGTTAAAAGAAGAAAACAGTAATTCGCTC
TTCTACCCGACAAAATCTGTTGAAGAATTGTTCGAAGTAAAAGATGAATAA

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10285.70 Da        Isoelectric Point: 5.7252

>AT51509 WP_048213818.1 NZ_CP010226:c1837281-1837012 [Escherichia coli]
MSALKKQRIDLRLNEDDKHMIEEAAAMTNQSISQFMVSTASERAAEVIDQHRRLLLNEESWNLVMDAITNPPVPNDRLKR
AANRLRELE

Download         Length: 270 bp

>AT51509 NZ_CP010226:c1837281-1837012 [Escherichia coli]
ATGTCAGCACTGAAAAAGCAGCGCATTGACCTGAGATTAAATGAAGACGACAAACACATGATCGAAGAAGCTGCAGCAAT
GACTAACCAGTCAATTTCGCAGTTCATGGTCAGTACCGCCTCAGAACGCGCTGCAGAAGTTATTGACCAGCACCGTCGTC
TGCTCCTCAATGAGGAGTCCTGGAACCTGGTTATGGATGCAATCACTAATCCGCCAGTACCGAATGACCGGCTGAAACGC
GCCGCTAACCGTCTCCGGGAACTGGAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A8B2S6X5

Antitoxin

Source ID Structure
AlphaFold DB A0A8B2S700

References