51459

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) TsxAB/Xre(antitoxin)
Location 36455..36956 Replicon plasmid A
Accession NZ_CP010220
Organism Escherichia coli strain M18

Toxin (Protein)


Gene name TsxA Uniprot ID A0A2S5RIK4
Locus tag RG62_RS27100 Protein ID WP_022645141.1
Coordinates 36455..36715 (+) Length 87 a.a.

Antitoxin (Protein)


Gene name TsxB Uniprot ID A0A2S5RIE4
Locus tag RG62_RS27105 Protein ID WP_022645142.1
Coordinates 36705..36956 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RG62_RS27050 32055..32216 + 162 WP_001434039.1 type I toxin-antitoxin system Hok family toxin -
RG62_RS27055 32366..32962 + 597 WP_000125164.1 hypothetical protein -
RG62_RS28515 32980..33264 - 285 WP_001162371.1 hypothetical protein -
RG62_RS27065 33338..34003 - 666 WP_000532148.1 P-loop NTPase -
RG62_RS27075 34600..34929 + 330 WP_000855121.1 hypothetical protein -
RG62_RS27085 35508..35831 + 324 WP_000427402.1 hypothetical protein -
RG62_RS27090 35872..36081 + 210 WP_000866037.1 hypothetical protein -
RG62_RS27095 36179..36403 + 225 WP_000814383.1 hypothetical protein -
RG62_RS27100 36455..36715 + 261 WP_022645141.1 hypothetical protein Toxin
RG62_RS27105 36705..36956 + 252 WP_022645142.1 transcriptional regulator Antitoxin
RG62_RS27120 37506..37784 + 279 WP_000545937.1 hypothetical protein -
RG62_RS27125 38172..38354 - 183 WP_001485010.1 hypothetical protein -
RG62_RS27130 38912..39094 + 183 WP_000833470.1 type II toxin-antitoxin system HicA family toxin -
RG62_RS27135 39119..39556 + 438 WP_000466318.1 type II toxin-antitoxin system HicB family antitoxin -
RG62_RS27140 39688..40008 - 321 WP_001401701.1 hypothetical protein -
RG62_RS28295 40342..40520 - 179 Protein_54 hypothetical protein -
RG62_RS27150 40731..41180 - 450 WP_052974092.1 hypothetical protein -
RG62_RS27155 41200..41907 - 708 WP_000108794.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - - 1..57582 57582


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

Cro/C1-type helix-turn-helix domain

(8-37)


Sequences

Toxin        


Download         Length: 87 a.a.        Molecular weight: 9876.23 Da        Isoelectric Point: 7.2030

>T51459 WP_022645141.1 NZ_CP010220:36455-36715 [Escherichia coli]
MKIRKGDRQYYLNKEGDTFHLVKRVKTFSKSATLGKTKATVKTVADLVFHEEAFDTIDFASDGLRENDKEIVSMMIQEMS
EEKNAN

Download         Length: 261 bp

>T51459 NZ_CP010220:36455-36715 [Escherichia coli]
ATGAAGATAAGAAAAGGTGACAGACAATATTATCTGAATAAAGAAGGTGATACGTTTCATCTGGTGAAGCGAGTAAAAAC
TTTTTCTAAGTCTGCGACTTTAGGAAAAACGAAAGCAACGGTGAAAACTGTTGCTGATCTTGTTTTTCATGAGGAGGCAT
TCGACACTATCGACTTTGCCAGCGACGGATTAAGAGAGAATGATAAAGAAATTGTTTCTATGATGATTCAGGAAATGAGC
GAGGAAAAAAATGCTAACTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9407.81 Da        Isoelectric Point: 9.8120

>AT51459 WP_022645142.1 NZ_CP010220:36705-36956 [Escherichia coli]
MLTENTPENIKRLRQKIGLTQKECAEIFSMSPRTWRRKEEPVGTASGTALTPVEFKFLLLLAGEHPDYVLCKRNKKNSDS
GSN

Download         Length: 252 bp

>AT51459 NZ_CP010220:36705-36956 [Escherichia coli]
ATGCTAACTGAAAACACACCAGAAAACATTAAGCGTCTGCGGCAAAAAATTGGCCTGACACAGAAAGAATGTGCCGAAAT
ATTTAGCATGAGTCCGCGTACCTGGCGTCGCAAAGAAGAACCTGTCGGAACTGCAAGCGGTACAGCACTTACACCAGTAG
AGTTTAAATTTCTTCTGCTTTTAGCTGGAGAACACCCTGATTATGTACTTTGTAAAAGGAATAAAAAGAATTCTGATTCT
GGAAGTAATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2S5RIK4

Antitoxin

Source ID Structure
AlphaFold DB A0A2S5RIE4

References