Detailed information of TA system TA051454

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 4934599..4935134
• Replicon: chromosome
• Accession: NZ_CP010219
• Organism: Escherichia coli strain M18

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: RG62_RS25820
• Protein ID: WP_001270421.1
• Coordinates: 4934847..4935134 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: L4J0V6
• Locus tag: RG62_RS25815
• Protein ID: WP_001132895.1
• Coordinates: 4934599..4934850 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RG62_RS25785	4930153..4931322	+	1170	WP_046201818.1	transposase	-
RG62_RS25790	4931362..4931535	+	174	Protein_4645	DUF4113 domain-containing protein	-
RG62_RS25795	4931676..4932638	-	963	WP_046201819.1	ParB/RepB/Spo0J family partition protein	-
RG62_RS25800	4932635..4933840	-	1206	WP_000817642.1	AAA family ATPase	-
RG62_RS25810	4934214..4934426	+	213	WP_000402944.1	hypothetical protein	-
RG62_RS25815	4934599..4934850	+	252	WP_001132895.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
RG62_RS25820	4934847..4935134	+	288	WP_001270421.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
RG62_RS25825	4935148..4935407	+	260	Protein_4651	hypothetical protein	-
RG62_RS25830	4935420..4935710	+	291	WP_046201820.1	hypothetical protein	-
RG62_RS28165	4935757..4936124	+	368	Protein_4653	DDE-type integrase/transposase/recombinase	-
RG62_RS25850	4936768..4937745	+	978	WP_000361612.1	RepB family plasmid replication initiator protein	-
RG62_RS25855	4938024..4938764	-	741	WP_046201823.1	tyrosine-type recombinase/integrase	-
RG62_RS25860	4938885..4939046	-	162	WP_064756529.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	pic	4835798..5080594	244796
	-	flank	IS/Tn	-	-	4939633..4940310	677

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-83)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11244.27 Da
• Isoelectric Point: 10.4703

>T51454 WP_001270421.1 NZ_CP010219:4934847-4935134 [Escherichia coli]
MRYQVKFREDALKEWQKLDKTIQQQFAKKLKKCCDNPHIPSAKLRGIKDCYKIKLRASGFRLVYQVIDEQLIIAVVAVGK
RERSDVYNLASERMR

Nucleotide

• Length: 288 bp

>T51454 NZ_CP010219:4934847-4935134 [Escherichia coli]
ATGAGGTATCAGGTAAAATTCAGGGAAGATGCGCTGAAAGAGTGGCAAAAACTGGACAAGACTATTCAGCAACAGTTTGC
GAAAAAGCTAAAAAAGTGCTGTGACAATCCGCATATTCCTTCCGCAAAACTGCGCGGGATAAAGGACTGCTACAAAATAA
AATTACGTGCGTCAGGTTTTCGCCTAGTCTATCAGGTGATTGACGAACAATTGATTATCGCTGTTGTAGCTGTGGGTAAA
CGTGAGCGCAGTGACGTTTATAATCTTGCCAGTGAAAGAATGAGATAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9178.47 Da
• Isoelectric Point: 4.2108

>AT51454 WP_001132895.1 NZ_CP010219:4934599-4934850 [Escherichia coli]
MPNIILSDTSASVSELKKNPMATVSAGDGFPVAILNRNQPAFYCVPAELYEKMLDALDDQELVKLVAERSNQPLHDVDLD
KYL

Nucleotide

• Length: 252 bp

>AT51454 NZ_CP010219:4934599-4934850 [Escherichia coli]
ATGCCTAACATTATTCTCAGTGATACAAGCGCCAGTGTCAGCGAGCTGAAGAAAAATCCGATGGCGACAGTCAGCGCCGG
TGATGGTTTCCCGGTCGCTATCCTGAACCGTAATCAGCCTGCTTTCTACTGTGTACCCGCAGAGCTGTACGAAAAGATGC
TCGATGCCCTGGACGATCAGGAACTGGTTAAACTGGTAGCCGAACGCAGCAACCAACCGCTGCATGATGTAGACCTGGAT
AAATACCTATGA

Structures

Antitoxin

• AlphaFold DB: L4J0V6