Detailed information of TA system TA051452

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 3964936..3965771
• Replicon: chromosome
• Accession: NZ_CP010219
• Organism: Escherichia coli strain M18

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: RG62_RS20645
• Protein ID: WP_049590183.1
• Coordinates: 3965394..3965771 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: RG62_RS20640
• Protein ID: WP_049590182.1
• Coordinates: 3964936..3965304 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RG62_RS20595	3961060..3961515	+	456	WP_065227643.1	hypothetical protein	-
RG62_RS20610	3961799..3962620	+	822	WP_073527751.1	DUF945 domain-containing protein	-
RG62_RS20620	3962959..3963438	+	480	WP_000706981.1	antirestriction protein	-
RG62_RS20625	3963454..3963930	+	477	WP_073527752.1	RadC family protein	-
RG62_RS20630	3963999..3964220	+	222	WP_000692323.1	DUF987 domain-containing protein	-
RG62_RS20635	3964239..3964886	+	648	WP_049590180.1	hypothetical protein	-
RG62_RS20640	3964936..3965304	+	369	WP_049590182.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
RG62_RS20645	3965394..3965771	+	378	WP_049590183.1	TA system toxin CbtA family protein	Toxin
RG62_RS20650	3965768..3966256	+	489	WP_000761700.1	hypothetical protein	-
RG62_RS20655	3966276..3966473	+	198	WP_000772024.1	DUF957 domain-containing protein	-
RG62_RS20660	3966570..3967418	+	849	WP_049590184.1	DUF4942 domain-containing protein	-
RG62_RS20665	3967511..3968830	-	1320	WP_073527753.1	site-specific integrase	-
RG62_RS20670	3969168..3969506	-	339	Protein_3732	LysR family transcriptional regulator	-
RG62_RS20675	3969557..3970513	-	957	WP_000121330.1	molybdenum cofactor insertion chaperone PaoD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	3962959..3975392	12433

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13984.01 Da
• Isoelectric Point: 9.6545

>T51452 WP_049590183.1 NZ_CP010219:3965394-3965771 [Escherichia coli]
MKTLPVLPGQSASSRPSPVEIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SAGAPSQLINSIDILRARRATGLMTRNNYRMVNNITRGKHPEAKR

Nucleotide

• Length: 378 bp

>T51452 NZ_CP010219:3965394-3965771 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGTCGGCCAGTTCACGCCCATCTCCTGTTGAAATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTATGGCCTTACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGGATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGTACCGACCAGCCGGGATTC
AGCGCAGGAGCCCCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGGAA
CAATTACAGAATGGTAAATAACATTACCCGGGGCAAGCATCCGGAGGCAAAACGATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13710.64 Da
• Isoelectric Point: 6.9371

>AT51452 WP_049590182.1 NZ_CP010219:3964936-3965304 [Escherichia coli]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRASIRGRFRDADAYHLDQAFPLLMKQLKLMLTSG
ELNPCHQHTVTLYARGLICEADTFGSCGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT51452 NZ_CP010219:3964936-3965304 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCAGACCGCGCCAGTATCAGAGGCCGGT
TCAGGGACGCAGATGCATACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGAAATTAATGCTCACTAGCGGT
GAACTGAATCCCTGCCATCAGCATACCGTCACGCTGTATGCCAGAGGGCTGATCTGCGAAGCCGACACCTTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA