Detailed information of TA system TA051445

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 1983216..1984051
• Replicon: chromosome
• Accession: NZ_CP010219
• Organism: Escherichia coli strain M18

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: RG62_RS10395
• Protein ID: WP_073527670.1
• Coordinates: 1983674..1984051 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A2Y0C7M1
• Locus tag: RG62_RS10390
• Protein ID: WP_047661876.1
• Coordinates: 1983216..1983584 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RG62_RS10355	1979998..1980231	+	234	WP_073527667.1	DUF905 family protein	-
RG62_RS10365	1980331..1981149	+	819	WP_073527668.1	DUF945 domain-containing protein	-
RG62_RS10370	1981240..1981725	+	486	WP_073527669.1	antirestriction protein	-
RG62_RS10375	1981741..1982217	+	477	WP_041124171.1	RadC family protein	-
RG62_RS10380	1982286..1982507	+	222	WP_047661878.1	DUF987 domain-containing protein	-
RG62_RS10385	1982522..1983166	+	645	WP_047661877.1	hypothetical protein	-
RG62_RS10390	1983216..1983584	+	369	WP_047661876.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
RG62_RS10395	1983674..1984051	+	378	WP_073527670.1	TA system toxin CbtA family protein	Toxin
RG62_RS27860	1984048..1984197	+	150	Protein_1890	hypothetical protein	-
RG62_RS27865	1984273..1984470	+	198	WP_087891818.1	DUF957 domain-containing protein	-
RG62_RS10405	1984555..1985400	+	846	WP_073527671.1	DUF4942 domain-containing protein	-
RG62_RS10420	1986808..1988351	+	1544	Protein_1894	EAL domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	gspM / gspL / gspK / gspJ / gspI / gspH / gspG / gspF / gspE / gspD / gspC	1933031..2051179	118148

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14106.04 Da
• Isoelectric Point: 7.7532

>T51445 WP_073527670.1 NZ_CP010219:1983674-1984051 [Escherichia coli]
MKTSPDTHVREASCCPSPVTIWQTLLSRLLDQHYGLTLNNTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGL
STCPRSQFINSIDILRARRATGLMTRSHYRTVNDITQGKHPEAKQ

Nucleotide

• Length: 378 bp

>T51445 NZ_CP010219:1983674-1984051 [Escherichia coli]
ATGAAAACATCACCCGATACACACGTACGGGAGGCATCGTGCTGCCCGTCTCCCGTCACCATCTGGCAGACTCTGCTCTC
CCGACTGCTGGACCAGCACTACGGCCTCACACTGAATAACACTCCATTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTCTGTGATGCAGTGAACTTTCTCGTTGAAAAATACGCCCTGGTGCGTACCGACCAGCCCGGGCTC
AGCACTTGTCCCCGCTCTCAGTTCATAAACAGCATCGATATCCTCCGGGCCCGCCGGGCAACAGGTCTGATGACCCGCAG
CCACTACAGAACGGTAAATGACATTACCCAGGGGAAGCATCCGGAGGCGAAACAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13660.30 Da
• Isoelectric Point: 5.4561

>AT51445 WP_047661876.1 NZ_CP010219:1983216-1983584 [Escherichia coli]
VSDSLHETNYPDDHNDRPWWGLPCTVTPCFGARLVQEGNQLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELSPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTSETKT

Nucleotide

• Length: 369 bp

>AT51445 NZ_CP010219:1983216-1983584 [Escherichia coli]
GTGTCAGACTCGCTCCATGAGACAAATTATCCCGACGACCATAACGACCGCCCCTGGTGGGGACTGCCCTGCACCGTGAC
ACCCTGTTTTGGCGCCCGTCTGGTGCAGGAAGGCAACCAGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCGGT
TCAGCGACGCGGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTCAGCCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCAGCTG
TGGTTACGTTTATCTGGCTGTTTATCCAACATCCGAAACGAAAACGTAA

Structures

Antitoxin

• AlphaFold DB: A0A2Y0C7M1