Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 295182..295820 Replicon chromosome
Accession NZ_CP010219
Organism Escherichia coli strain M18

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag RG62_RS01565 Protein ID WP_000813794.1
Coordinates 295182..295358 (+) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag RG62_RS01570 Protein ID WP_001270286.1
Coordinates 295404..295820 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RG62_RS01545 290801..292015 - 1215 WP_071609973.1 BenE family transporter YdcO -
RG62_RS01550 292068..292604 + 537 WP_000429155.1 DNA-binding transcriptional regulator SutR -
RG62_RS01555 292677..294638 + 1962 WP_001442195.1 23S rRNA 5-hydroxycytidine C2501 synthase -
RG62_RS01560 294730..294960 - 231 WP_000494244.1 YncJ family protein -
RG62_RS01565 295182..295358 + 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
RG62_RS01570 295404..295820 + 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
RG62_RS01575 295899..297305 + 1407 WP_000760663.1 PLP-dependent aminotransferase family protein -
RG62_RS01580 297550..298695 + 1146 WP_000047424.1 ABC transporter substrate-binding protein -
RG62_RS01585 298713..299726 + 1014 WP_001542893.1 ABC transporter ATP-binding protein -
RG62_RS01590 299727..300668 + 942 WP_001251313.1 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 289613..290761 1148


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T51431 WP_000813794.1 NZ_CP010219:295182-295358 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T51431 NZ_CP010219:295182-295358 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT51431 WP_001270286.1 NZ_CP010219:295404-295820 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT51431 NZ_CP010219:295404-295820 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCGTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAAGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAATTTGCACCATGCGACAAAAATCGACGCCGTCCAGCTTGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References