Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) parDE/RHH(antitoxin)
Location 58986..59467 Replicon plasmid A
Accession NZ_CP010214
Organism Escherichia coli strain M15

Toxin (Protein)


Gene name parE Uniprot ID -
Locus tag RG61_RS26920 Protein ID WP_073519917.1
Coordinates 58986..59201 (-) Length 72 a.a.

Antitoxin (Protein)


Gene name parD Uniprot ID -
Locus tag RG61_RS26925 Protein ID WP_073519918.1
Coordinates 59198..59467 (-) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RG61_RS26885 54669..55922 - 1254 WP_073519913.1 HlyD family efflux transporter periplasmic adaptor subunit -
RG61_RS26890 56025..56660 - 636 WP_073519914.1 CPBP family intramembrane metalloprotease -
RG61_RS26895 56665..56865 - 201 WP_073519915.1 hypothetical protein -
RG61_RS29220 56890..57252 - 363 WP_071597330.1 hypothetical protein -
RG61_RS29225 57486..57569 - 84 Protein_67 maturase -
RG61_RS26905 57727..58266 + 540 WP_077897976.1 recombinase family protein -
RG61_RS26910 58259..58531 + 273 WP_046201741.1 hypothetical protein -
RG61_RS26915 58535..58834 - 300 WP_073519916.1 hypothetical protein -
RG61_RS26920 58986..59201 - 216 WP_073519917.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
RG61_RS26925 59198..59467 - 270 WP_073519918.1 hypothetical protein Antitoxin
RG61_RS26935 59679..59810 + 132 WP_071595370.1 replication protein RepA4 -
RG61_RS26940 59826..60020 - 195 WP_073519921.1 replication protein -
RG61_RS26945 60382..61210 - 829 Protein_75 incFII family plasmid replication initiator RepA -
RG61_RS26950 61203..61277 - 75 WP_001365705.1 RepA leader peptide Tap -
RG61_RS26955 61344..61492 + 149 Protein_77 replication protein RepA -
RG61_RS26960 61509..61766 - 258 WP_073519922.1 replication regulatory protein RepA -
RG61_RS26965 61967..62116 - 150 WP_001312851.1 type I toxin-antitoxin system Hok family toxin -
- 62165..62221 + 57 NuclAT_1 - -
- 62165..62221 + 57 NuclAT_1 - -
- 62165..62221 + 57 NuclAT_1 - -
- 62165..62221 + 57 NuclAT_1 - -
RG61_RS26970 62172..62414 + 243 WP_071996372.1 hypothetical protein -
RG61_RS26975 62394..62984 - 591 WP_032334802.1 DUF2726 domain-containing protein -
RG61_RS26980 63022..63231 - 210 WP_001299729.1 hemolysin expression modulator Hha -
RG61_RS26985 63277..63738 - 462 WP_077898159.1 thermonuclease family protein -
RG61_RS26990 63981..64193 - 213 WP_032334799.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - faeI / faeH / faeF / faeE / faeD / faeC / pic 1..146496 146496


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(7-64)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 72 a.a.        Molecular weight: 8017.24 Da        Isoelectric Point: 6.4468

>T51414 WP_073519917.1 NZ_CP010214:c59201-58986 [Escherichia coli]
MMEIFCTMLASQDRKRIREYIAEQNLMAAIELDELIGYSASSLAGQPYKGRNGRVEGTRELVIHPHFCSGL

Download         Length: 216 bp

>T51414 NZ_CP010214:c59201-58986 [Escherichia coli]
ATGATGGAGATATTCTGTACTATGCTGGCCAGCCAGGACAGGAAGCGCATTCGTGAGTACATTGCGGAGCAGAACCTGAT
GGCCGCCATTGAACTGGACGAACTGATTGGTTATTCAGCCAGCAGCCTGGCCGGGCAGCCGTATAAAGGCCGCAACGGTC
GTGTGGAAGGTACCCGGGAACTGGTTATTCACCCCCATTTTTGTTCTGGTTTATGA

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10310.62 Da        Isoelectric Point: 6.4710

>AT51414 WP_073519918.1 NZ_CP010214:c59467-59198 [Escherichia coli]
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQHNTWFRDQVEEGRQQLERGDVLPHDMVESSAAAWRD
EMSRKVAGK

Download         Length: 270 bp

>AT51414 NZ_CP010214:c59467-59198 [Escherichia coli]
ATGGCACAGGTTAATATGAGTTTAAGAATCGACGCTGAACTGAAGGATGCTTTTATGGCCGCTGCAAAAAGCATGGACCG
TAATGGCTCTCAGTTAATCCGGGATTTTATGCGCCAGACCGTTGAACGGCAGCATAATACCTGGTTCCGTGACCAGGTTG
AGGAAGGACGTCAGCAACTCGAGCGCGGTGATGTGCTTCCCCATGACATGGTCGAATCCTCTGCTGCAGCATGGCGGGAT
GAAATGAGCAGGAAGGTCGCCGGTAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References